从Qualea属植物甲醇提取物中鉴定鞣花酸衍生物。

Identification of ellagic acid derivatives in methanolic extracts from Qualea species.

作者信息

Nasser Ana L M, Carli Camila B A, Rodrigues Clenilson M, Maia Danielle C G, Carlos Iracilda Z, Eberlin Marcos N, Hiruma-Lima Clélia A, Vilegas Wagner

机构信息

UNESP, São Paulo State University, Instituto de Química de Araraquara, C. Postal 355, 14801-970, Araraquara, SP, Brazil.

出版信息

Z Naturforsch C J Biosci. 2008 Nov-Dec;63(11-12):794-800. doi: 10.1515/znc-2008-11-1203.

DOI:10.1515/znc-2008-11-1203

PMID:19227825

Abstract

The methanolic extract from the barks of the medicinal plant Qualea parviflora (Vochysiaceae) was fractionated by column chromatography over silica gel followed by gel permeation over Sephadex LH-20 to give 3,3'-di-O-methylellagic acid-4-O-beta-D-glucopyranoside (1), 3-O-methylellagic acid-4'-O-alpha-L-rhamnopyranoside (2), 3,3',4-tri-O-methylellagic acid-4'-O-beta-D-glucopyranoside (3), and 3,3'-di-O-methylellagic acid (4), together with triterpenes and saponins. We also performed comparative analyses among this species and Q. grandiflora and Q. multiflora using high-pressure liquid chromatography. The biological assays showed that, when compared to the standard ellagic acid, compounds 1-4 are less cytotoxic but have a lower capacity of stimulating murine peritoneal macrophages to release nitric oxide and tumoural-alpha necrose factor.

摘要

对药用植物小花夸勒木（Vochysiaceae科）树皮的甲醇提取物进行硅胶柱色谱分离，随后通过葡聚糖凝胶LH - 20进行凝胶渗透，得到3,3'-二 - O - 甲基鞣花酸 - 4 - O - β - D - 吡喃葡萄糖苷（1）、3 - O - 甲基鞣花酸 - 4'- O - α - L - 鼠李吡喃糖苷（2）、3,3',4 - 三 - O - 甲基鞣花酸 - 4'- O - β - D - 吡喃葡萄糖苷（3）和3,3'-二 - O - 甲基鞣花酸（4），以及三萜类化合物和皂苷。我们还使用高压液相色谱法对该物种与大花夸勒木和多花夸勒木进行了比较分析。生物学测定表明，与标准鞣花酸相比，化合物1 - 4的细胞毒性较小，但刺激小鼠腹腔巨噬细胞释放一氧化氮和肿瘤坏死因子 - α的能力较低。