[荔枝核含药血清对HepG2细胞增殖及凋亡的影响]
[Effect of Semen Litchi containing serum on proliferation and apoptosis of HepG2 cells].
作者信息
Xiong Ai-Hua, Shen Wen-Juan, Xiao Liu-Ying, Lv Jun-Hua
机构信息
School of Pharmacy, Jinan University, Guangzhou 510632, China.
出版信息
Zhong Yao Cai. 2008 Oct;31(10):1533-6.
OBJECTIVE
To observe the effect of Semen Litchi containing serum on proliferation and apoptosis of HepG2 cells.
METHODS
The Semen litchi or CTX containing serum and control serum were prepared by serologic pharmacology method. MTT assay was used to observe the proliferation inhibition rate of HepG2 cells after incubated with different kinds of drug's containing serum. Nuclear morphological features of HepG2 cells were detected by fluorescencemicroscopy after staining with Hochest33258. The apoptosis rate of HepG2 cells in each group was detected by flow cytometry.
RESULTS
The cell viability and the apoptosis rate of HepG2 cells in Semen Litchi containing serum groups were higher than that of control group, and the results of fluorescencemicroscopy observation showed the nuclear morphological change of apoptosis.
CONCLUSION
Semen Litchi can inhibit the proliferation of HepG2 cells, the acting mechanism may be concerned with cell apoptosis.
目的
观察荔枝核含药血清对HepG2细胞增殖及凋亡的影响。
方法
采用血清药理学方法制备荔枝核含药血清、环磷酰胺含药血清及对照血清。采用MTT法观察不同含药血清作用后HepG2细胞的增殖抑制率。用Hochest33258染色后通过荧光显微镜检测HepG2细胞的核形态特征。采用流式细胞术检测各组HepG2细胞的凋亡率。
结果
荔枝核含药血清组HepG2细胞的细胞活力及凋亡率高于对照组，荧光显微镜观察结果显示有凋亡的核形态改变。
结论
荔枝核可抑制HepG2细胞增殖，作用机制可能与细胞凋亡有关。
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