健康个体肠道紧密连接通透性与全身电阻之间的关联：一种假说。

Association between intestinal tight junction permeability and whole-body electrical resistance in healthy individuals: a hypothesis.

作者信息

Valentini Luzia, Eggers Jonathan, Ockenga Johann, Haas Verena K, Bühner Sabine, Winklhofer-Roob Brigitte M, Hengstermann Susanne, Sinn Brigitte, Weigel Andrea, Norman Kristina, Pirlich Matthias, Lochs Herbert

机构信息

Department Gastroenterology, Hepatology and Endocrinology, Charité Universitätsmedizin Berlin, Berlin, Germany.

出版信息

Nutrition. 2009 Jun;25(6):706-14. doi: 10.1016/j.nut.2008.11.033. Epub 2009 Feb 20.

DOI:10.1016/j.nut.2008.11.033

PMID:19230613

Abstract

OBJECTIVE

Intestinal permeability describes non-carrier-mediated modes of transport through the intestinal epithelium. Wrist-ankle bioimpedance analysis (BIA) is a standard method to determine body composition based on the measurements of whole-body electrical resistance and reactance values. The present report deals with the coincidentally observed associations between permeability results and electrical raw values of BIA and their subsequent reproduction in a larger group of individuals.

METHODS

Tetrapolar wrist-ankle BIA was performed on day 1 in the initial sample (12 women, 36 +/- 11 y of age) and the validation sample (36 healthy subjects, 26 women and 10 men, 35 +/- 14 y of age). Intestinal permeability tests (lactulose and mannitol) were implemented within 1 wk thereafter. Wrist-ankle electrical resistance plus electrical resistance between current-conducting electrodes and voltage-sensing electrodes (Rtotal) was measured at 5 kHz and 100 kHz.

RESULTS

Permeability and bioimpedance raw values were normal, indicating normal tight junction permeability and normal hydration. Lactulose correlated to R(50total) in the initial sample (rho = 0.639, P = 0.025) and in the validation sample (rho = 0.673, P < 0.001). Weaker associations to R(50total) were observed with mannitol (rho = 0.381, P = 0.008) and lactulose/mannitol (rho = 0.369, P = 0.010) in the total group of individuals. Regression analyses demonstrated that R(50total) alone accounted for 41.3% of the variance in lactulose permeability.

CONCLUSION

The nature of the observed positive association between intestinal tight junction permeability and whole-body electrical resistance is unclear. We hypothesize that regulation involving submolecular mechanisms based on the principles of quantum physics might have caused the observed association. Such coherent mechanisms might possibly play a role in basal physiologic regulation in humans.

摘要

目的

肠道通透性描述了通过肠上皮的非载体介导的转运模式。腕踝生物电阻抗分析（BIA）是一种基于全身电阻和电抗值测量来确定身体成分的标准方法。本报告探讨了通透性结果与BIA电原始值之间偶然观察到的关联，以及随后在更大规模个体群体中的重现情况。

方法

在初始样本（12名女性，年龄36±11岁）和验证样本（36名健康受试者，26名女性和10名男性，年龄35±14岁）的第1天进行四极腕踝BIA。此后1周内进行肠道通透性测试（乳果糖和甘露醇）。在5kHz和100kHz下测量腕踝电阻以及导电电极和电压感应电极之间的电阻（Rtotal）。

结果

通透性和生物电阻抗原始值正常，表明紧密连接通透性正常且水合作用正常。在初始样本（rho = 0.639，P = 0.025）和验证样本（rho = 0.673，P < 0.001）中，乳果糖与R(50total)相关。在个体总数中，甘露醇（rho = 0.381，P = 0.008）和乳果糖/甘露醇（rho = 0.369，P = 0.010）与R(50total)的关联较弱。回归分析表明，仅R(50total)就占乳果糖通透性方差的41.3%。