Velard Frédéric, Laurent-Maquin Dominique, Guillaume Christine, Bouthors Sylvie, Jallot Edouard, Nedelec Jean-Marie, Belaaouaj Abderrazzaq, Laquerriere Patrice
INSERM, UMR-S 926, CHU de Reims, IFR 53, URCA, 1 avenue du Maréchal Juin, 51095 Reims, Cedex, France.
Acta Biomater. 2009 Jun;5(5):1708-15. doi: 10.1016/j.actbio.2009.01.008. Epub 2009 Jan 21.
Hydroxyapatite (HA) is widely used as a bone substitute or coating biomaterial in bone diseases or prosthesis metal parts. The release of HA particles induces an inflammatory response and, if uncontrolled, could result in implant loss. Among the hallmarks of such inflammatory response is early recruitment of the polymorphonuclear cells (PMNs). The purpose of this work is to investigate the response of PMNs following exposure to HA in terms of secreted mediators. Our study shows that HA particles increase the release of pro-inflammatory mediators such as interleukin-1alpha, as well as chemotactic factors such as interleukin-8, macrophage inflammatory protein-1alpha and macrophage inflammatory protein-1beta. HA also induces an increase in matrix metalloproteinase 9 expression. Taken together, our data demonstrate for the first time that HA is capable of activating PMNs, a phenomenon that could potentially contribute to the onset of implant-associated inflammation.
羟基磷灰石（HA）作为骨替代物或涂层生物材料被广泛应用于骨疾病治疗或假体金属部件。HA颗粒的释放会引发炎症反应，若不加以控制，可能导致植入物失效。这种炎症反应的特征之一是多形核细胞（PMN）的早期募集。本研究旨在探讨PMN在接触HA后分泌介质方面的反应。我们的研究表明，HA颗粒会增加促炎介质如白细胞介素-1α的释放，以及趋化因子如白细胞介素-8、巨噬细胞炎性蛋白-1α和巨噬细胞炎性蛋白-1β的释放。HA还会诱导基质金属蛋白酶9表达增加。综上所述，我们的数据首次证明HA能够激活PMN，这一现象可能会导致植入物相关炎症的发生。
