Stücker Sina, Koßlowksi Franziska, Buchholz Adrian, Schwab Andrea, Halm-Pozniak Agnieszka, Lohmann Christoph H, Bertrand Jessica
Department of Orthopaedic Surgery, Otto-von-Guericke-University, Magdeburg, Germany.
Front Cell Dev Biol. 2025 Mar 19;13:1535530. doi: 10.3389/fcell.2025.1535530. eCollection 2025.
Calcification of articular tissues is commonly observed in later osteoarthritis (OA) stages and can be caused by basic calcium phosphate (BCP) or calcium pyrophosphate (CPP) crystals. Calcification, particularly CPP deposition, has recently been associated with inflammation and cellular senescence. Investigating this association, we analyzed the concentration of various inflammatory mediators in synovial fluid and synovial membrane of OA patients in relation to calcification and the different crystal types.
Synovial fluid was collected from OA patients during joint replacement surgery. Cytokine concentrations were measured using magnetic bead-based multiplex assay using Luminex® technology. Radiographs were used to determine and grade calcification of the knee joint and involved calcium crystal types were identified via Raman spectroscopy.
Synovial fluid of patients with radiological calcification showed elevated levels of multiple cytokines (IL-10, IL-15, IL-1ra, GM-CSF), chemokines (IL-8, MCP-1, MIP-1b) and growth factors (PDGF-AB/BB, VEGF). Crystal differentiation revealed higher synovial fluid concentrations of IL-15, IL-1ra, IL-10, GM-CSF, PDGF-AB/BB and MIP-1b in patients with CPP- compared to BCP-calcified cartilage.
We show an elevated cytokine profile in synovial fluid of patients with radiological calcification that may be linked to CPP depositison in cartilage.
关节组织钙化在骨关节炎（OA）后期阶段较为常见，可能由碱性磷酸钙（BCP）或焦磷酸钙（CPP）晶体引起。钙化，尤其是CPP沉积，最近被认为与炎症和细胞衰老有关。为了研究这种关联，我们分析了OA患者滑液和滑膜中各种炎症介质的浓度与钙化及不同晶体类型的关系。
在关节置换手术期间从OA患者收集滑液。使用基于磁珠的多重分析方法（采用Luminex®技术）测量细胞因子浓度。通过X线片确定膝关节钙化情况并进行分级，通过拉曼光谱鉴定所涉及的钙晶体类型。
放射学检查有钙化的患者滑液中多种细胞因子（IL-10、IL-15、IL-1ra、GM-CSF）、趋化因子（IL-8、MCP-1、MIP-1b）和生长因子（PDGF-AB/BB、VEGF）水平升高。晶体鉴别显示，与BCP钙化软骨的患者相比，CPP钙化软骨的患者滑液中IL-15、IL-1ra、IL-10、GM-CSF、PDGF-AB/BB和MIP-1b的浓度更高。
我们发现放射学检查有钙化的患者滑液中细胞因子水平升高，这可能与软骨中的CPP沉积有关。
