Sridharan Indumathi, Kim Taeyoung, Wang Rong
Department of Biological, Illinois Institute of Technology, Chicago, 60616, USA.
Biochem Biophys Res Commun. 2009 Apr 17;381(4):508-12. doi: 10.1016/j.bbrc.2009.02.072. Epub 2009 Feb 20.
The lineage selection in human embryonic stem cell (hESC) differentiation relies on both the growth factors and small molecules in the media and the physical characteristics of the micro-environment. In this work, we utilized various materials, including the collagen-carbon nanotube (collagen/CNT) composite material, as cell culture matrices to examine the impact of matrix properties on hESC differentiation. Our AFM analysis indicated that the collagen/CNT formed rigid fibril bundles, which polarized the growth and differentiation of hESCs, resulting in more than 90% of the cells to the ectodermal lineage in Day 3 in the media commonly used for spontaneous differentiation. We also observed the differentiated cells followed the coarse alignment of the collagen/CNT matrix. The research not only revealed the responsiveness of hESCs to matrix properties, but also provided a simple yet efficient way to direct the hESC differentiation, and imposed the potential of forming neural-cell based bio-devices for further applications.
人类胚胎干细胞（hESC）分化过程中的谱系选择既依赖于培养基中的生长因子和小分子，也依赖于微环境的物理特性。在这项工作中，我们利用了包括胶原-碳纳米管（collagen/CNT）复合材料在内的各种材料作为细胞培养基质，以研究基质特性对hESC分化的影响。我们的原子力显微镜（AFM）分析表明，胶原/CNT形成了刚性纤维束，使hESCs的生长和分化极化，在常用于自发分化的培养基中，第三天超过90%的细胞分化为外胚层谱系。我们还观察到分化细胞沿着胶原/CNT基质的大致排列方向生长。该研究不仅揭示了hESCs对基质特性的反应性，还提供了一种简单而有效的方法来指导hESC分化，并为进一步应用形成基于神经细胞的生物装置带来了潜力。
