用于人类多能干细胞维持和分化的微纳工程技术
Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation.
作者信息
Esfahani Sajedeh Nasr, Resto Irizarry Agnes M, Xue Xufeng, Lee Samuel Byung-Deuk, Shao Yue, Fu Jiangping
机构信息
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Engineering Mechanics, Tsinghua University, Beijing, China.
出版信息
Nano Today. 2021 Dec;41. doi: 10.1016/j.nantod.2021.101310. Epub 2021 Oct 6.
Human pluripotent stem cells (hPSCs) are a promising source of cells for cell replacement-based therapies as well as modeling human development and diseases . However, achieving fate control of hPSC with a high yield and specificity remains challenging. The fate specification of hPSCs is regulated by biochemical and biomechanical cues in their environment. Driven by this knowledge, recent exciting advances in micro/nanoengineering have been leveraged to develop a broad range of tools for the generation of extracellular biomechanical and biochemical signals that determine the behavior of hPSCs. In this review, we summarize such micro/nanoengineered technologies for controlling hPSC fate and highlight the role of biochemical and biomechanical cues such as substrate rigidity, surface topography, and cellular confinement in the hPSC-based technologies that are on the horizon.
人类多能干细胞（hPSC）是基于细胞替代疗法以及模拟人类发育和疾病的有前景的细胞来源。然而，以高产率和特异性实现对hPSC的命运控制仍然具有挑战性。hPSC的命运特化受其环境中的生化和生物力学线索调控。受这一认识的推动，微纳工程领域最近取得了令人兴奋的进展，已被用于开发一系列工具，以产生决定hPSC行为的细胞外生物力学和生化信号。在本综述中，我们总结了此类用于控制hPSC命运的微纳工程技术，并强调了生化和生物力学线索，如底物刚度、表面形貌和细胞限制，在即将出现的基于hPSC的技术中的作用。
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