通过定点诱变对埃罗霉素糖基转移酶ElmGT的脱氧糖转移进行调控。
Modulation of deoxysugar transfer by the elloramycin glycosyltransferase ElmGT through site-directed mutagenesis.
作者信息
Ramos Angelina, Olano Carlos, Braña Alfredo F, Méndez Carmen, Salas José A
机构信息
Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
出版信息
J Bacteriol. 2009 Apr;191(8):2871-5. doi: 10.1128/JB.01747-08. Epub 2009 Feb 20.
Abstract
The glycosyltransferase ElmGT from Streptomyces olivaceus is involved in the biosynthesis of the antitumor drug elloramycin, and it has been shown to possess a broad deoxysugar recognition pattern, being able to transfer different l- and d-deoxysugars to 8-demethyl-tetracenomycin C, the elloramycin aglycone. Site-directed mutagenesis in residues L309 and N312, located in the alpha/beta/alpha motif within the nucleoside diphosphate-sugar binding region, can be used to modulate the substrate flexibility of ElmGT, making it more precise for transfer of specific deoxysugars.
摘要
来自橄榄链霉菌的糖基转移酶ElmGT参与抗肿瘤药物埃罗霉素的生物合成，并且已证明它具有广泛的脱氧糖识别模式，能够将不同的L-和D-脱氧糖转移到埃罗霉素苷元8-去甲基-四并苯霉素C上。位于核苷二磷酸糖结合区域内α/β/α基序中的L309和N312残基的定点诱变可用于调节ElmGT的底物灵活性，使其对特定脱氧糖的转移更精确。
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