糖基转移酶结构生物学及其在糖基化催化剂设计中的作用。
Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.
机构信息
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
Curr Opin Biotechnol. 2011 Dec;22(6):800-8. doi: 10.1016/j.copbio.2011.04.013. Epub 2011 May 16.
Abstract
Glycosyltransferases (GTs) are ubiquitous in nature and are required for the transfer of sugars to a variety of important biomolecules. This essential enzyme family has been a focus of attention from both the perspective of a potential drug target and a catalyst for the development of vaccines, biopharmaceuticals and small molecule therapeutics. This review attempts to consolidate the emerging lessons from Leloir (nucleotide-dependent) GT structural biology studies and recent applications of these fundamentals toward rational engineering of glycosylation catalysts.
摘要
糖基转移酶（GTs）在自然界中普遍存在，是将糖转移到各种重要生物分子所必需的。这种重要的酶家族一直是潜在药物靶点和疫苗、生物制药和小分子治疗药物开发的催化剂的关注焦点。本文综述尝试整合 Leloir（核苷酸依赖）GT 结构生物学研究的新成果，以及这些基础研究在糖基化催化剂理性设计中的最新应用。
相似文献
1
Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.糖基转移酶结构生物学及其在糖基化催化剂设计中的作用。
Curr Opin Biotechnol. 2011 Dec;22(6):800-8. doi: 10.1016/j.copbio.2011.04.013. Epub 2011 May 16.
2
Recent progress in synthesis of carbohydrates with sugar nucleotide-dependent glycosyltransferases.糖核苷酸依赖的糖基转移酶合成碳水化合物的最新进展。
Curr Opin Chem Biol. 2021 Apr;61:81-95. doi: 10.1016/j.cbpa.2020.10.007. Epub 2020 Dec 10.
3
Glycosyltransferase engineering for carbohydrate synthesis.用于碳水化合物合成的糖基转移酶工程
Biochem Soc Trans. 2016 Feb;44(1):129-42. doi: 10.1042/BST20150200.
4
Leloir glycosyltransferases of natural product C-glycosylation: structure, mechanism and specificity.天然产物 C-糖基化的 Leloir 糖基转移酶：结构、机制和特异性。
Biochem Soc Trans. 2020 Aug 28;48(4):1583-1598. doi: 10.1042/BST20191140.
5
Recent Progress in Structural Studies on the GT-C Superfamily of Protein Glycosyltransferases.GT-C 家族蛋白糖基转移酶的结构研究进展。
Subcell Biochem. 2021;96:259-271. doi: 10.1007/978-3-030-58971-4_6.
6
Expanding the promiscuity of a natural-product glycosyltransferase by directed evolution.通过定向进化扩展天然产物糖基转移酶的底物宽泛性。
Nat Chem Biol. 2007 Oct;3(10):657-62. doi: 10.1038/nchembio.2007.28. Epub 2007 Sep 9.
7
Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.刺孢霉素生物合成早期糖基化步骤的生化与结构解析
Chem Biol. 2008 Aug 25;15(8):842-53. doi: 10.1016/j.chembiol.2008.06.011.
8
Biotechnological advances in UDP-sugar based glycosylation of small molecules.基于 UDP-糖的小分子糖基化的生物技术进展。
Biotechnol Adv. 2015 Mar-Apr;33(2):288-302. doi: 10.1016/j.biotechadv.2015.02.005. Epub 2015 Feb 16.
9
Chapter 12. The power of glycosyltransferases to generate bioactive natural compounds.第12章：糖基转移酶生成生物活性天然化合物的能力。
Methods Enzymol. 2009;458:309-33. doi: 10.1016/S0076-6879(09)04812-5.
10
Recombinant E. coli prototype strains for in vivo glycorandomization.用于体内糖随机化的重组大肠杆菌原型菌株。
ACS Chem Biol. 2011 Jan 21;6(1):95-100. doi: 10.1021/cb100267k. Epub 2010 Oct 22.
引用本文的文献
1
Crystal structure of the fungal mannosyltransferase Och1 reveals active site primed for N-glycan binding.真菌甘露糖基转移酶Och1的晶体结构揭示了为N-聚糖结合做好准备的活性位点。
PLoS One. 2025 Jul 31;20(7):e0329259. doi: 10.1371/journal.pone.0329259. eCollection 2025.
2
Genome-Wide Identification of UGT Gene Family and Functional Analysis of in .基因组范围内UGT基因家族的鉴定及[物种名称]中[基因名称]的功能分析 （你提供的原文不完整，推测是这样补充完整后的表述，你可根据实际情况调整）
Int J Mol Sci. 2025 May 18;26(10):4832. doi: 10.3390/ijms26104832.
3
Glycosyltransferases: glycoengineers in human milk oligosaccharide synthesis and manufacturing.糖基转移酶：人乳寡糖合成与制造中的糖基工程酶
Front Mol Biosci. 2025 Apr 30;12:1587602. doi: 10.3389/fmolb.2025.1587602. eCollection 2025.
4
The metabolic enzyme GYS1 condenses with NONO/p54 in the nucleus and spatiotemporally regulates glycogenesis and myogenic differentiation.代谢酶GYS1在细胞核中与NONO/p54凝聚，并在时空上调节糖原生成和肌源性分化。
Cell Death Differ. 2025 Apr 8. doi: 10.1038/s41418-025-01509-4.
5
β-Carotene alleviates substrate inhibition caused by asymmetric cooperativity.β-胡萝卜素可减轻由不对称协同作用引起的底物抑制。
Nat Commun. 2025 Mar 29;16(1):3065. doi: 10.1038/s41467-025-58259-7.
6
Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.利用挖掘出的糖基转移酶通过级联反应高效合成红景天苷。
Bioprocess Biosyst Eng. 2025 Jun;48(6):939-949. doi: 10.1007/s00449-025-03153-1. Epub 2025 Mar 27.
7
Genome-Wide Identification and Analysis of Glycosyltransferases in .. 中糖基转移酶的全基因组鉴定与分析
Microorganisms. 2024 Dec 11;12(12):2551. doi: 10.3390/microorganisms12122551.
8
Re-evaluation of the -Glucosyltransferase IroB Illuminates Its Ability to -Glucosylate Non-native Triscatecholate Enterobactin Mimics.对β-葡萄糖基转移酶IroB的重新评估揭示了其对非天然三儿茶酚肠杆菌素模拟物进行β-葡萄糖基化的能力。
Biochemistry. 2025 Jan 7;64(1):224-237. doi: 10.1021/acs.biochem.4c00581. Epub 2024 Dec 24.
9
Identification of glycosyltransferases mediating 2-O-arabinopyranosyl and 2-O-galactosyl substitutions of glucuronosyl side chains of xylan.鉴定木聚糖糖醛酸侧链 2-O-阿拉伯糖基和 2-O-半乳糖基取代的糖基转移酶。
Plant J. 2024 Oct;120(1):234-252. doi: 10.1111/tpj.16983. Epub 2024 Aug 15.
10
Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in .小麦全基因组UDP糖基转移酶对中国小麦花叶病毒抗性响应的分析与鉴定 。（原英文文本表述不太完整，推测补充完整后的意思大致如此）
Viruses. 2024 Mar 22;16(4):489. doi: 10.3390/v16040489.
本文引用的文献
1
Substrate and metal ion promiscuity in mannosylglycerate synthase.甘露糖甘油酸合酶的底物和金属离子混杂性。
J Biol Chem. 2011 Apr 29;286(17):15155-64. doi: 10.1074/jbc.M110.199844. Epub 2011 Feb 2.
2
Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins.半胱氨酸 S-糖基化，一种在糖肽类细菌素中发现的新型翻译后修饰。
FEBS Lett. 2011 Feb 18;585(4):645-50. doi: 10.1016/j.febslet.2011.01.023. Epub 2011 Jan 18.
3
Structure of human O-GlcNAc transferase and its complex with a peptide substrate.人 O-连接的 N-乙酰氨基葡萄糖转移酶的结构及其与肽底物的复合物。
Nature. 2011 Jan 27;469(7331):564-7. doi: 10.1038/nature09638. Epub 2011 Jan 16.
4
Sublancin is not a lantibiotic but an S-linked glycopeptide.Sublancin 不是一种类细菌素，而是一种 S 连接的糖肽。
Nat Chem Biol. 2011 Feb;7(2):78-80. doi: 10.1038/nchembio.509. Epub 2011 Jan 2.
5
Trapping and characterization of covalent intermediates of mutant retaining glycosyltransferases.突变型保留糖基转移酶的共价中间体的捕获和表征。
Glycobiology. 2011 May;21(5):547-52. doi: 10.1093/glycob/cwq190. Epub 2010 Nov 22.
6
Recombinant E. coli prototype strains for in vivo glycorandomization.用于体内糖随机化的重组大肠杆菌原型菌株。
ACS Chem Biol. 2011 Jan 21;6(1):95-100. doi: 10.1021/cb100267k. Epub 2010 Oct 22.
7
Structural basis for glucose-6-phosphate activation of glycogen synthase.葡萄糖-6-磷酸激活糖原合酶的结构基础。
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17563-8. doi: 10.1073/pnas.1006340107. Epub 2010 Sep 27.
8
Acceptor substrate discrimination in phosphatidyl-myo-inositol mannoside synthesis: structural and mutational analysis of mannosyltransferase Corynebacterium glutamicum PimB'.磷酸肌醇甘露糖基转移酶 Corynebacterium glutamicum PimB'的底物接受基识别：结构和突变分析。
J Biol Chem. 2010 Nov 26;285(48):37741-52. doi: 10.1074/jbc.M110.165407. Epub 2010 Sep 15.
9
Functional differentiation of the glycosyltransferases that contribute to the chemical diversity of bioactive flavonol glycosides in grapevines (Vitis vinifera).葡萄（Vitis vinifera）中生物活性类黄酮醇糖苷化学多样性形成的糖基转移酶的功能分化。
Plant Cell. 2010 Aug;22(8):2856-71. doi: 10.1105/tpc.110.074625. Epub 2010 Aug 6.
10
Cysteine-to-serine mutants dramatically reorder the active site of human ABO(H) blood group B glycosyltransferase without affecting activity: structural insights into cooperative substrate binding.半胱氨酸到丝氨酸突变体显著改变了人 ABO(H)血型 B 糖基转移酶的活性位点，而不影响其活性：协同底物结合的结构见解。
J Mol Biol. 2010 Sep 17;402(2):399-411. doi: 10.1016/j.jmb.2010.07.036. Epub 2010 Jul 23.
Zotero 插件