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Probing the activation of the replicative origin of broad host-range plasmid R1162 with Tus, the E.coli anti-helicase protein.

作者信息

Zhou H S, Byrd C, Meyer R J

机构信息

Department of Microbiology, University of Texas, Austin 78712.

出版信息

Nucleic Acids Res. 1991 Oct 11;19(19):5379-83. doi: 10.1093/nar/19.19.5379.

DOI:10.1093/nar/19.19.5379
PMID:1923822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328902/
Abstract

The E.coli Tus protein is an anti-helicase involved in the termination of chromosome replication. The binding site for this protein, ter, was cloned into derivatives of the broad host-range plasmid R1162. The ter site caused the orientation-specific termination of plasmid replication fork movement in cell extracts containing Tus. Plasmids were constructed so that two sites for initiation of R1162 replication flanked the iteron-containing domain of the origin. In these plasmids, the site next to the AT-rich region within the iteron-containing domain was more active. In addition, when ter was placed between the more active site and the iterons, initiation of replication from this site was specifically inhibited. The data support a model for entry of the essential, plasmid-encoded helicase at one side of the direct repeats, and for its movement primarily in one direction away from these repeats to activate the initiation sites for DNA replication.

摘要

相似文献

1
Probing the activation of the replicative origin of broad host-range plasmid R1162 with Tus, the E.coli anti-helicase protein.
Nucleic Acids Res. 1991 Oct 11;19(19):5379-83. doi: 10.1093/nar/19.19.5379.
2
The interaction of RepC initiator with iterons in the replication of the broad host-range plasmid RSF1010.广宿主范围质粒RSF1010复制过程中RepC起始蛋白与迭代子的相互作用。
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3
Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro.大肠杆菌Tus蛋白在体外可阻止DNA复制叉的前进。
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4
A multifunctional plasmid-encoded replication initiation protein both recruits and positions an active helicase at the replication origin.一种多功能的质粒编码复制起始蛋白在复制起点募集并定位活性解旋酶。
Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8692-7. doi: 10.1073/pnas.1532393100. Epub 2003 Jun 30.
5
Equilibrium, kinetic, and footprinting studies of the Tus-Ter protein-DNA interaction.Tus-Ter蛋白与DNA相互作用的平衡、动力学及足迹分析研究。
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6
DNA sequence requirements for interaction of the RK2 replication initiation protein with plasmid origin repeats.RK2复制起始蛋白与质粒起源重复序列相互作用的DNA序列要求。
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7
An essential iteron-binding protein required for plasmid R1162 replication induces localized melting within the origin at a specific site in AT-rich DNA.质粒R1162复制所需的一种必需的迭代子结合蛋白可在富含AT的DNA中的特定位点诱导起始点内的局部解链。
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Interactions of plasmid-encoded replication initiation proteins with the origin of DNA replication in the broad host range plasmid RK2.质粒编码的复制起始蛋白与广宿主范围质粒RK2中DNA复制起点的相互作用。
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本文引用的文献

1
Vertical dye-buoyant density gradients for rapid analysis and preparation of plasmid DNA.用于快速分析和制备质粒DNA的垂直染料浮力密度梯度
Anal Biochem. 1981 Nov 15;118(1):191-3. doi: 10.1016/0003-2697(81)90177-9.
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Proc Natl Acad Sci U S A. 1984 Feb;81(3):654-8. doi: 10.1073/pnas.81.3.654.
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Properties of R1162, a broad-host-range, high-copy-number plasmid.R1162的特性，一种广宿主范围、高拷贝数的质粒。
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5
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.质粒ColE1作为用于DNA克隆和扩增的分子载体。
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6
Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min.抑制从大肠杆菌染色体末端区域退出的复制叉发生在两个相隔5分钟的位点。
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759-63. doi: 10.1073/pnas.84.7.1759.
7
The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.大肠杆菌染色体的末端区域包含两个独立的位点，它们表现出对复制的极性抑制。
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8
DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.DNA合成在质粒R1162复制起点内的两个位置起始。
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9
A consensus sequence of three DNA replication terminus sites on the E. coli chromosome is highly homologous to the terR sites of the R6K plasmid.大肠杆菌染色体上三个DNA复制终止位点的共有序列与R6K质粒的terR位点高度同源。
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10
Core sequence of two separable terminus sites of the R6K plasmid that exhibit polar inhibition of replication is a 20 bp inverted repeat.R6K质粒两个可分离的末端位点的核心序列呈现出复制的极性抑制，该核心序列是一个20个碱基对的反向重复序列。
Cell. 1988 Aug 12;54(4):515-23. doi: 10.1016/0092-8674(88)90073-6.