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DNA合成在质粒R1162复制起点内的两个位置起始。

DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.

作者信息

Lin L S, Meyer R J

机构信息

Department of Microbiology, University of Texas, Austin 78712.

出版信息

Nucleic Acids Res. 1987 Oct 26;15(20):8319-31. doi: 10.1093/nar/15.20.8319.

DOI:10.1093/nar/15.20.8319
PMID:3313280
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306362/
Abstract

DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted repeat. An analysis of deletions suggests that both start positions must be present for synthesis. A model describing possible early events in replication of plasmid R1162 is presented.

摘要

广宿主范围质粒R1162的DNA合成从两个位置起始，这两个位置位于一个大的（40个碱基对的茎，40个碱基对的环）反向重复序列两侧。每个起始点位于一个高度保守但方向相反的10个碱基对序列内。从这两个位置开始的合成在中间的反向重复序列内汇聚。缺失分析表明，两个起始位置对于合成都是必需的。本文提出了一个描述质粒R1162复制中可能早期事件的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/aa66cb96ff8e/nar00264-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/c672f4a60137/nar00264-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/7896f4a4d8c6/nar00264-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/71cba100535d/nar00264-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/aa66cb96ff8e/nar00264-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/c672f4a60137/nar00264-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/7896f4a4d8c6/nar00264-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/71cba100535d/nar00264-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681e/306362/aa66cb96ff8e/nar00264-0209-a.jpg

相似文献

1
DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.DNA合成在质粒R1162复制起点内的两个位置起始。
Nucleic Acids Res. 1987 Oct 26;15(20):8319-31. doi: 10.1093/nar/15.20.8319.
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Deletion of sites for initiation of DNA synthesis in the origin of broad host-range plasmid R1162.
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Directly repeated, 20-bp sequence of plasmid R1162 DNA is required for replication, expression of incompatibility, and copy-number control.质粒R1162 DNA的20个碱基对直接重复序列是复制、不相容性表达和拷贝数控制所必需的。
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本文引用的文献

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Nucleic Acids Res. 1982 Aug 11;10(15):4687-702. doi: 10.1093/nar/10.15.4687.
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Plasmid P1 replication: negative control by repeated DNA sequences.
绘制广宿主范围质粒R1162（RSF1010）编码的引发酶上的IV型分泌信号图谱。
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Comparative biology of IncQ and IncQ-like plasmids.IncQ及类IncQ质粒的比较生物学
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Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon.从嗜酸细菌嗜热嗜酸硫杆菌中分离出一种新的广宿主范围类IncQ质粒pTC-F14并对该质粒复制子进行分析。
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