Miyazaki Teruo, Bouscarel Bernard, Ikegami Tadashi, Honda Akira, Matsuzaki Yasushi
The George Washington University, District of Columbia, USA.
Adv Exp Med Biol. 2009;643:293-303. doi: 10.1007/978-0-387-75681-3_30.
Taurine plays a protective role against free radicals and toxins in various cells and tissues. However, the effect of taurine on hepatic injury and fibrosis developed by activated hepatic stellate cells (HSC) and myofibroblast-like cells is not fully understood. We investigated the effects of taurine on the hepatic fibrogenesis and damage in rats and isolated HSC. Rats were divided into a normal and two CCl4-induced liver damage (LD) groups, one untreated and the other maintained for 5 weeks on a 2% taurine diet. The HSC isolated from a normal rat were cultured either for a day only or for an additional 3-6 days with approximately 50 mM taurine. LD rats maintained on the taurine diet were resistant to CCl4-induced loss of taurine from the liver. The liver of the LD rats were also protected against histological damage, fibrosis, significant reductions in oxidative stress markers (LPO and 8-OHdG) and hepatic fibrogenic factors (TGF-beta1 mRNA, hydroxyproline, alpha-SMA). Proliferation, oxidative stress, and fibrogenesis were significantly inhibited in HSC by treatment with taurine. Thus, supplementation with taurine should be considered as a therapeutic approach to lessen the severity of oxidative stress-induced liver injury and hepatic fibrosis.
牛磺酸对各种细胞和组织中的自由基和毒素具有保护作用。然而，牛磺酸对活化的肝星状细胞（HSC）和成肌纤维细胞样细胞所导致的肝损伤和纤维化的影响尚未完全明确。我们研究了牛磺酸对大鼠肝纤维化形成及损伤以及分离的肝星状细胞的影响。将大鼠分为正常组和两个四氯化碳诱导的肝损伤（LD）组，一组不进行处理，另一组在含2%牛磺酸的饮食条件下维持5周。从正常大鼠分离出的肝星状细胞，要么仅培养一天，要么再用约50 mM牛磺酸额外培养3 - 6天。以含牛磺酸饮食维持的肝损伤大鼠对四氯化碳诱导的肝脏牛磺酸丢失具有抗性。肝损伤大鼠的肝脏在组织学损伤、纤维化、氧化应激标志物（脂质过氧化和8 - 羟基脱氧鸟苷）以及肝纤维化因子（转化生长因子 - β1 mRNA、羟脯氨酸、α - 平滑肌肌动蛋白）显著降低方面也受到保护。用牛磺酸处理可显著抑制肝星状细胞的增殖、氧化应激和纤维化形成。因此，补充牛磺酸应被视为减轻氧化应激诱导的肝损伤和肝纤维化严重程度的一种治疗方法。
