Fakhrullin Rawil F, Zamaleeva Alsu I, Morozov Mikhail V, Tazetdinova Diana I, Alimova Farida K, Hilmutdinov Albert K, Zhdanov Renat I, Kahraman Mehmet, Culha Mustafa
Department of Biochemistry, Kazan State University, Kremlevskaya 18, Kazan 420008, Tatarstan, Russian Federation.
Langmuir. 2009 Apr 21;25(8):4628-34. doi: 10.1021/la803871z.
We report the layer-by-layer coating of living fungi cells (Saccharomyces cerevisiae and Trichoderma asperellum) with polyelectrolytes poly(allylamine hydrochloride)/sodium poly(styrene sulfonate) and bovine serum albumin/DNA and citrate-stabilized gold and silver nanoparticles. It was found that the nanoparticles were effectively incorporated between oppositely charged polyelectrolyte layers, modifying the topography and the roughness of cell walls. The formation of large aggregates of nanoparticles on the cell walls of encapsulated cells was shown. It was found that the encapsulated cells preserved their viability and the shells were soft enough to allow the growth of mycelium. The surface-enhanced Raman scattering (SERS) was used to investigate the biochemical environments of the gold and silver nanoparticles immobilized on the surface of T. asperellum conidia. The SERS spectra from encapsulated conidia and polyelectrolytes indicate that both gold and silver nanoparticles interact with cell walls from different locations, and nanoparticle-polyelectrolyte interaction is limited. The approach described in this paper might have potential applications in modification of living cells.
我们报道了用聚电解质聚（烯丙胺盐酸盐）/聚苯乙烯磺酸钠、牛血清白蛋白/DNA以及柠檬酸盐稳定的金和银纳米颗粒对活真菌细胞（酿酒酵母和棘孢木霉）进行逐层包覆。研究发现，纳米颗粒有效地掺入带相反电荷的聚电解质层之间，改变了细胞壁的形貌和粗糙度。结果表明，在包封细胞的细胞壁上形成了大量纳米颗粒聚集体。研究发现，包封的细胞保持了其活力，并且外壳足够柔软，能够让菌丝体生长。利用表面增强拉曼散射（SERS）研究了固定在棘孢木霉分生孢子表面的金和银纳米颗粒的生化环境。来自包封分生孢子和聚电解质的SERS光谱表明，金和银纳米颗粒均从不同位置与细胞壁相互作用，且纳米颗粒-聚电解质相互作用有限。本文所述方法可能在活细胞修饰方面具有潜在应用。
