Kawaguchi Sachie, Hagiwara Akira, Suzuki Mamoru
Department of Otorhinolaryngology, Tokyo Medical University, Tokyo, Japan.
Acta Otolaryngol. 2008 Nov;128(11):1173-7. doi: 10.1080/00016480801901675.
The single nucleotide polymorphism (SNP) 190 G/C in HSP70-1 (HSPA1A) was found to be a factor associated with Meniere's disease (MD). It is suggested that SNP 190 G/C could be scientific evidence to prove the relation between MD and stress as a trigger factor.
Although it is widely known that MD might be caused by psychological stress, definitive scientific evidence has not been established. To understand an association between stress and MD, we analyzed the SNP of HSP70-1 (HSPA1A) in patients with MD.
We enrolled 49 patients given a diagnosis MD, consisting of 20 men and 29 women. Controls were 100 normal subjects. We isolated genomic DNA from peripheral leukocytes with the PAX gene Blood DNA kit (Qiagen), and determined the SNP of HSPA1A. All subjects were investigated as regards hearing level and vertigo attacks, and were evaluated as to the profile of mood states (POMS) concerning psychological stress.
A SNP located on position 190 was found within a regulatory region in HSPA1A, and the frequency of having at least one 190C allele was significantly higher in the MD patients than the controls (p < 0.001).
发现热休克蛋白70-1（HSP70-1，即HSPA1A）中的单核苷酸多态性（SNP）190 G/C是与梅尼埃病（MD）相关的一个因素。提示SNP 190 G/C可能是证明MD与作为触发因素的应激之间关系的科学证据。
尽管众所周知MD可能由心理应激引起，但尚未确立确凿的科学证据。为了解应激与MD之间的关联，我们分析了MD患者中HSP70-1（HSPA1A）的SNP。
我们纳入了49例诊断为MD的患者，其中男性20例，女性29例。对照组为100名正常受试者。我们使用PAX基因血液DNA试剂盒（Qiagen）从外周血白细胞中分离基因组DNA，并确定HSPA1A的SNP。对所有受试者进行听力水平和眩晕发作调查，并就心理应激方面的情绪状态剖面图（POMS）进行评估。
在HSPA1A的一个调控区域内发现了位于190位的一个SNP，MD患者中至少有一个190C等位基因的频率显著高于对照组（p < 0.001）。
