UV 固定厚涂片法可提高金胺染色的敏感性。
UV-fixed-thick-blotch preparation improves sensitivity of auramine staining.
机构信息
Microbiology Laboratory, Biotechnology Clinic Management Unit, Torrecardenas Hospital Complex, Almería, Spain.
出版信息
J Clin Microbiol. 2013 Oct;51(10):3400-2. doi: 10.1128/JCM.00974-13. Epub 2013 Jul 17.
Abstract
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.
摘要
我们在此描述了一种非常简单的改良金胺染色程序，即制备 UV 固定厚涂片，这使我们的总灵敏度达到 0.82（592 份抗酸杆菌 [AFB] 阳性标本/722 份初始分枝杆菌培养阳性呼吸道标本），分枝杆菌复合群的灵敏度为 0.93（526 份 AFB 阳性标本/564 份培养阳性标本），非结核分枝杆菌的灵敏度为 0.42（66 份 AFB 阳性标本/158 份培养阳性标本）。
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