使用甲磺酸三卡因（MS222）对爬行动物实施安乐死。
Use of tricaine methanesulfonate (MS222) for euthanasia of reptiles.
作者信息
Conroy C J, Papenfuss T, Parker J, Hahn N E
机构信息
Museum of Vertebrate Zoology, University of California, Berkeley, California, USA.
出版信息
J Am Assoc Lab Anim Sci. 2009 Jan;48(1):28-32.
Abstract
Tricaine methanesulfonate (MS222) injected into the intracoelomic cavity of reptiles was evaluated as a chemical euthanasia method. Three western fence lizards, 2 desert iguanas, 4 garter snakes, and 6 geckos were euthanized by intracoelomic injection of 250 to 500 mg/kg of 0.7% to 1% sodium-bicarbonate-buffered MS222 solution followed by intracoelomic injection of 0.1 to 1.0 ml unbuffered 50% (v/v) MS222 solution. A simple 2-stage protocol for euthanasia of reptiles by using MS222 is outlined. In addition, the conditions for safe use of MS222 are discussed. MS222 offers an alternative to sodium pentobarbital for euthanasia of reptiles.
摘要
将甲磺酸三卡因（MS222）注入爬行动物的体腔，作为一种化学安乐死方法进行了评估。通过向三只西部围栏蜥蜴、两只沙漠鬣蜥、四条束带蛇和六只壁虎的体腔内注射250至500毫克/千克的0.7%至1%碳酸氢钠缓冲MS222溶液，随后再向体腔内注射0.1至1.0毫升未缓冲的50%（体积/体积）MS222溶液，使其安乐死。概述了一种使用MS222对爬行动物进行安乐死的简单两阶段方案。此外，还讨论了安全使用MS222的条件。MS222为爬行动物的安乐死提供了一种替代戊巴比妥钠的方法。
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本文引用的文献
1
Nociception after intraperitoneal injection of a sodium pentobarbitone formulation with and without lidocaine in rats quantified by expression of neuronal c-fos in the spinal cord--a preliminary study.
Lab Anim. 2007 Apr;41(2):197-203. doi: 10.1258/002367707780378140.
2
Evaluation of Hypothermia for Anesthesia in Reptiles and Amphibians.
ILAR J. 1995;37(4):186-190. doi: 10.1093/ilar.37.4.186.
3
Crocodilian anesthesia.
Vet Clin North Am Exot Anim Pract. 2001 Jan;4(1):119-45, vii. doi: 10.1016/s1094-9194(17)30054-3.
4
Retinal toxicity associated with occupational exposure to the fish anesthetic MS-222.
Am J Ophthalmol. 1997 Dec;124(6):843-4. doi: 10.1016/s0002-9394(14)71705-2.
5
Recommendations for euthanasia of experimental animals: Part 2. DGXT of the European Commission.
Lab Anim. 1997 Jan;31(1):1-32. doi: 10.1258/002367797780600297.
6
Tropical fish medicine. Anesthesia in fish.
Vet Clin North Am Small Anim Pract. 1988 Mar;18(2):317-30. doi: 10.1016/s0195-5616(88)50034-7.
7
Studies on the comparative pharmacology and selective toxicity of tricaine methanesulfonate: metabolism as a basis of the selective toxicity in poikilotherms.
J Pharmacol Exp Ther. 1976 Sep;198(3):695-708.
8
Anesthetic effects and elimination of tricaine methanesulphonate (MS-222) in terrestial vertebrates.
Comp Biochem Physiol C Comp Pharmacol. 1976;55(1):37-41. doi: 10.1016/0306-4492(76)90009-5.
9
Tricaine methanesulfonate--1. pH and its effects on anesthetic potency.
Comp Biochem Physiol C Comp Pharmacol. 1976;54(1):13-7. doi: 10.1016/0306-4492(76)90018-6.
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