Ramachandran Reshma, Ford Emerald, Gomaa Basant, Abdelhamed Hossam
Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS 39762, USA.
Pathogens. 2024 Apr 11;13(4):310. doi: 10.3390/pathogens13040310.
Infection with a causative agent of enteric septicemia of catfish, threatens profitable catfish production through inventory losses. We previously demonstrated that trans-cinnamaldehyde (TC) enhances the survival of catfish following infection. The present study was conducted to investigate catfish immune responses to TC feeding and infection. The expression of 13 proinflammatory, innate, and adaptive immune-related genes was evaluated over time in two sets of experiments using real-time polymerase chain reaction (PCR). In the first experiment, catfish were fed a basal diet with or without TC supplementation, while in the second they were fed a TC-supplemented or normal diet followed by infection with . The catfish group infected with and fed a TC-diet showed significant changes in the expression of innate and adaptive immune-related genes compared to control group. At 21 and 28 days post-infection, recovered fish showed significant increases in the expression of IgM in the anterior kidney and spleen. These results suggest that the supplemental dietary intake of TC can improve the immune status of catfish via engaging innate and adaptive immune responses and the production of memory cells in immunocompetent tissues. Together, this study provides an important foundation for the potential application of TC as an antimicrobial alternative in aquaculture.
感染鲶鱼肠道败血症的病原体，会因库存损失而威胁到鲶鱼养殖的盈利。我们之前证明了反式肉桂醛（TC）能提高鲶鱼感染后的存活率。本研究旨在调查鲶鱼对TC投喂和感染的免疫反应。在两组实验中，使用实时聚合酶链反应（PCR）随时间评估了13种促炎、先天和适应性免疫相关基因的表达。在第一个实验中，鲶鱼被投喂添加或未添加TC的基础饲料，而在第二个实验中，它们被投喂添加TC的饲料或正常饲料，随后感染[病原体名称缺失]。与对照组相比，感染[病原体名称缺失]并投喂TC饲料的鲶鱼组在先天和适应性免疫相关基因的表达上有显著变化。在感染后21天和28天，恢复的鱼在前肾和脾脏中IgM的表达显著增加。这些结果表明，通过参与先天和适应性免疫反应以及在免疫活性组织中产生记忆细胞，饲料中补充TC可以改善鲶鱼的免疫状态。总之，本研究为TC作为水产养殖中抗菌替代品的潜在应用提供了重要基础。
