1st Plastic & Reconstructive Surgery Clinic, Ankara Training and Research Hospital, Ankara, Turkey.
J Plast Reconstr Aesthet Surg. 2010 Apr;63(4):705-10. doi: 10.1016/j.bjps.2009.01.018. Epub 2009 Feb 25.
Proanthocyanidins are potent natural antioxidants which belong to a class of polyphenols. Proanthocyanidin-rich extracts are prepared from grape seeds. The effect of grape seed proanthocyanidin extract (GSPE) on the viability of abdominal skin flaps exposed to warm ischaemia and subsequent reperfusion were studied in 40 male Wistar rats. In the control group (group I; n=20), rats were fed with standard, non-purified rat diet, and the study group received GSPE 100 mgkg(-1) per day 1 week prior to surgery and 1 week following surgery. Abdominal island flaps were elevated in both the groups and subjected to 8h of warm ischaemia, followed by reperfusion. Mean flap survival areas in groups I (control group) and II (treatment group) were calculated to be 58.3%+/-11.72 and 81.0%+/-11.88, respectively. Flap survival on day 7 was significantly higher in group II compared to group I (p<0.01). Histopathological semi-quantitative analysis of the specimens revealed infiltration by polymorphonuclear leucocytes, oedema formation and necrosis in group I, whereas neo-vascularisation and fibrosis were the prominent findings in group II.
原花青素是一种强效的天然抗氧化剂，属于多酚类物质。原花青素富含提取物来自葡萄籽。本研究旨在探讨葡萄籽原花青素提取物（GSPE）对腹部皮瓣暴露于温热缺血及再灌注后活性的影响。40 只雄性 Wistar 大鼠随机分为对照组（I 组，n=20）和 GSPE 治疗组（II 组，n=20）。对照组大鼠给予标准、非纯化的大鼠饲料，GSPE 治疗组大鼠在术前 1 周和术后 1 周每天给予 100mg/kg GSPE。两组大鼠均抬高腹部岛状皮瓣，缺血 8 小时，然后再灌注。I 组（对照组）和 II 组（治疗组）的平均皮瓣存活面积分别为 58.3%+/-11.72 和 81.0%+/-11.88。与 I 组相比，II 组皮瓣在第 7 天的存活面积明显更高（p<0.01）。组织病理学半定量分析显示，I 组标本中有多形核白细胞浸润、水肿形成和坏死，而 II 组标本中则有新生血管形成和纤维化。
