Aydogan Hakan, Gurlek Ali, Parlakpinar Hakan, Askar Ibrahim, Bay-Karabulut Aysun, Aydogan Nilay, Fariz Alpay, Acet Ahmet
Department of Plastic and Reconstructive Surgery, Inonu University, Medical Faculty, T. Ozal Medical Center, TR 44069 Malatya, Turkey.
J Plast Reconstr Aesthet Surg. 2007;60(5):563-8. doi: 10.1016/j.bjps.2006.01.030. Epub 2006 May 11.
Reperfusion injury is a phenomenon that occurs when tissues are subjected to ischaemia for a variable period of time, after which they are reperfused. Many factors have been implicated in the cause of reperfusion injury including free radicals and neutrophils. Caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE) is an active component of propolis from honeybee; it has anti-inflammatory and immunomodulatory properties, and protective effects against ischaemia-reperfusion (I/R) injury. We investigated the effects of CAPE on the survival of skin flaps in the rat.
Eighteen Wistar rats were used, and randomly divided into three groups (n=6 rats each group): the control group (Group 1), ethanol group (Group 2), and CAPE group (Group 3). A caudally based rectangular flap, 3x10 cm in size, was elevated on the back of the rat, according to the method described by Khouri and colleagues. The flap was sutured back into its original place. In the control group, saline 0.2 ml/day was given intraperitoneally (i.p.). Five percent ethanol 0.2 ml/day was administered i.p. in the ethanol group, and CAPE 50 micromol/kg/day i.p. in the CAPE group. To observe the effects of CAPE, levels of malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were measured from extracted skin tissue. Flap viability was evaluated seven days after the initial operation, measuring necrotic areas of flaps and total flap areas.
MDA and NO levels were significantly decreased in CAPE group; and however, GSH, GSH-Px, and SOD enzyme activities were significantly increased in CAPE group. We believed that the CAPE had beneficial effects to improve the survival of skin flaps since it has antioxidative and anti-inflammatory properties, and protective effects against I/R injury.
再灌注损伤是一种组织在经历一段可变时长的缺血后再灌注时发生的现象。许多因素被认为与再灌注损伤的成因有关，包括自由基和中性粒细胞。咖啡酸（3,4 - 二羟基肉桂酸）苯乙酯（CAPE）是蜜蜂蜂胶的一种活性成分；它具有抗炎和免疫调节特性，以及对缺血 - 再灌注（I/R）损伤的保护作用。我们研究了CAPE对大鼠皮瓣存活的影响。
使用18只Wistar大鼠，随机分为三组（每组n = 6只大鼠）：对照组（第1组）、乙醇组（第2组）和CAPE组（第3组）。按照Khouri及其同事描述的方法，在大鼠背部掀起一个大小为3×10 cm的尾部基底矩形皮瓣。将皮瓣缝合回其原位。对照组腹腔内注射（i.p.）0.2 ml/天的生理盐水。乙醇组腹腔内注射5%乙醇0.2 ml/天，CAPE组腹腔内注射50 μmol/kg/天的CAPE。为观察CAPE的作用，从提取的皮肤组织中测量丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH - Px）和超氧化物歧化酶（SOD）的水平。在初次手术后7天评估皮瓣活力，测量皮瓣坏死面积和皮瓣总面积。
CAPE组MDA和NO水平显著降低；然而，CAPE组GSH、GSH - Px和SOD酶活性显著增加。我们认为CAPE具有改善皮瓣存活的有益作用，因为它具有抗氧化和抗炎特性以及对I/R损伤的保护作用。
