Bozkurt Mehmet, Kapi Emin, Kulahci Yalcin, Gedik Ercan, Ozekinci Selver, Isik Fatma Birgul, Celik Yusuf, Selcuk Caferi Tayyar, Kuvat Samet Vasfi
Department of Plastic, Reconstructive and Aesthetic Surgery and Burn Center, Dicle University Medical Faculty , Diyarbakir , Turkey and Kartal Dr. Lufti Kirdar Training and Research Hospital, Istanbul, Turkey.
J Plast Surg Hand Surg. 2014 Feb;48(1):44-50. doi: 10.3109/2000656X.2013.800527. Epub 2013 Jun 4.
Free radicals are chemicals that play roles in the etio-pathogenesis of ischaemia-reperfusion injury. Various antioxidants have been used in an attempt to mitigate the damage induced by these chemicals. In the present study, the antioxidative effects of grape seed extract (proanthocyanidin), tomato extract (lycopene), and vitamin C (ascorbic acid) on a composite re-established-flow inferior epigastric artery based rectus abdominis muscle-skin flap model on which experimental ischaemia was induced were investigated. The rats have been administered antioxidants for 2 weeks prior to the surgery and for 2 more weeks thereafter. Macroscopic, histopathological, and biochemical analyses were carried out at the decision of the experiment. It was found that flap skin island necrosis was significantly reduced in the proanthocyanidin, lycopene, vitamin C groups (p < 0.001). Statistical analyses showed significant decreases in inflammation, oedema, congestion, and granulation tissue in the proanthocyanidin and lycopene groups compared to the vitamin C and control groups (p < 0.001). When the viability rates of fat and muscle tissues were examined, significant improvements were found in the proanthocyanidin and lycopene groups in comparison to the other groups (p < 0.001). Serum antioxidant capacity measurements revealed significant differences in the lycopene group compared to all other groups (p < 0.001). It is concluded that lycopene and proanthocyanidin are protective antioxidants in rat composite muscle-skin flap ischaemia-reperfusion models.
自由基是在缺血再灌注损伤的病因发病机制中起作用的化学物质。人们已使用各种抗氧化剂来减轻这些化学物质所诱导的损伤。在本研究中，研究了葡萄籽提取物（原花青素）、番茄提取物（番茄红素）和维生素C（抗坏血酸）对建立了复合再灌注的腹壁下动脉腹直肌肌皮瓣模型（该模型诱导了实验性缺血）的抗氧化作用。在手术前2周及术后再2周给大鼠施用抗氧化剂。在实验结束时进行宏观、组织病理学和生化分析。发现原花青素、番茄红素、维生素C组的皮瓣皮岛坏死显著减少（p<0.001）。统计分析表明，与维生素C组和对照组相比，原花青素组和番茄红素组的炎症、水肿、充血和肉芽组织显著减少（p<0.001）。检查脂肪和肌肉组织的存活率时，发现原花青素组和番茄红素组与其他组相比有显著改善（p<0.001）。血清抗氧化能力测量显示，番茄红素组与所有其他组相比有显著差异（p<0.001）。得出的结论是，在大鼠复合肌皮瓣缺血再灌注模型中，番茄红素和原花青素是具有保护作用的抗氧化剂。
