Bernbom Nete, Jelle Birthe, Brogren Carl-Henrik, Vogensen Finn K, Nørrung Birgit, Licht Tine Rask
Department of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
Int J Food Microbiol. 2009 Apr 15;130(3):251-7. doi: 10.1016/j.ijfoodmicro.2009.02.003. Epub 2009 Feb 11.
The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods. The culturable microbiota was in all treatments dominated by lactic acid bacteria and coliforms and no changes in the rat commensal microbiota were detected after ingestion of either of the two L. plantarum strains as determined by both culturable methods and molecular methods (DGGE). Both strains were detected in the faeces after ingestion. Pediocin PA-1 did not mediate changes of the rat microbiota, and a biological assay indicated that the bacteriocin was degraded or inactivated during passage through the intestine.
细菌素片球菌素PA-1有作为食品生物防腐剂的潜在用途，了解其对肠道共生微生物群的影响对于评估与使用生物防腐剂培养物相关的消费者风险很重要。通过选择性培养和分子方法，研究了摄入（i）产生片球菌素PA-1的植物乳杆菌DDEN 11007、（ii）质粒消除的片球菌素阴性植物乳杆菌DDEN 12305或（iii）这两种菌株中任何一种的上清液对人源化微生物群（HMA）大鼠肠道微生物群组成的影响。在所有处理中，可培养的微生物群均以乳酸菌和大肠菌群为主，通过可培养方法和分子方法（变性梯度凝胶电泳，DGGE）测定，摄入这两种植物乳杆菌菌株中的任何一种后，未检测到大鼠共生微生物群的变化。摄入后在粪便中检测到了这两种菌株。片球菌素PA-1并未介导大鼠微生物群的变化，一项生物学试验表明，该细菌素在通过肠道的过程中被降解或失活。
