Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Innovation Centre of Food Nutrition and Human Health (Beijing), Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
Innovation Centre of Food Nutrition and Human Health (Beijing), Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, People's Republic of China (ORCID: https://orcid.org/0000-0002-6061-1781 [H.Z.]).
J Food Prot. 2020 Oct 1;83(10):1693-1700. doi: 10.4315/JFP-19-587.
The quorum-sensing regulation of class II bacteriocin (AcH) synthesis in Lactobacillus plantarum subsp. plantarum Zhang-LL was studied. No detectable inhibition zone was formed by the supernatant of L. plantarum subsp. plantarum Zhang-LL culture in skim milk (SM) with an inoculum size of 7 × 102 CFU/mL after incubation for 36 h. Hence, this culture system was used to investigate the induced regulation mechanism of bacteriocin production in L. plantarum subsp. plantarum Zhang-LL. Bacteriocin production by this bacterium in SM medium was induced by treatment with inactivated culture supernatant from de Man Rogosa Sharpe (MRS) medium (supernatant-MRS). Pediocin AcH encoded by the papA gene in a plasmid in strain Zhang-LL was the inducer present in supernatant-MRS. This is the first report of the role of pediocin AcH in the quorum-sensing regulation of class II bacteriocin synthesis. The mRNA of the papA, papB, papC, and papD genes involved in bacteriocin synthesis by strain Zhang-LL in SM medium was upregulated significantly after being induced by pediocin AcH. This study offers the first evidence that the ABT40_05745, ABT40_05750, and ABT40_11975 components of two-component systems in L. plantarum subsp. plantarum Zhang-LL are involved in the induced regulation of AcH bacteriocin production.
本研究旨在探讨植物乳杆菌亚种植物亚种张-LL 中 II 类细菌素（AcH）合成的群体感应调控。在脱脂乳（SM）培养基中，当接种量为 7×102 CFU/mL 并培养 36 h 后，张-LL 培养物的上清液未形成可检测的抑菌圈。因此，该培养系统被用于研究植物亚种植物亚种张-LL 中细菌素产生的诱导调控机制。该细菌在 SM 培养基中的细菌素产量可通过处理来自德氏乳杆菌培养基（MRS）的失活培养上清液（上清液-MRS）来诱导。张-LL 菌株质粒上编码 pediocin AcH 的 papA 基因是上清液-MRS 中存在的诱导物。这是首次报道 pediocin AcH 在 II 类细菌素合成的群体感应调控中的作用。在被 pediocin AcH 诱导后，张-LL 菌株在 SM 培养基中合成细菌素的 papA、papB、papC 和 papD 基因的 mRNA 显著上调。本研究首次证明，植物乳杆菌亚种植物亚种张-LL 中两个组分系统的 ABT40_05745、ABT40_05750 和 ABT40_11975 参与了 AcH 细菌素产生的诱导调控。
