Ravyts F, Vrancken G, D'Hondt K, Vasilopoulos C, De Vuyst L, Leroy F
Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
Int J Food Microbiol. 2009 Aug 31;134(1-2):89-95. doi: 10.1016/j.ijfoodmicro.2009.02.006. Epub 2009 Feb 12.
Five species of meat-borne, coagulase-negative staphylococci were screened for their in vitro production of 3-methyl-1-butanol. The highest production level was encountered for Staphylococcus sciuri alphaSG2, despite its poor growth. With respect to Staphylococcus species that are generally applied in sausage starter cultures, production of 3-methyl-1-butanol was higher with Staphylococcus xylosus 3PA6 than with Staphylococcus carnosus 833. Mathematical modelling was used to link the kinetics of 3-methyl-1-butanol production by S. xylosus 3PA6 and S. carnosus 833 in meat simulation medium to bacterial growth and environmental factors, in casu temperature and pH. The specific production rate of 3-methyl-1-butanol was about ten times higher for S. xylosus 3PA6 than for S. carnosus 833, indicating a higher production rate per amount of biomass. This explains the higher concentrations of 3-methyl-1-butanol in the medium with S. xylosus 3PA6, despite its poorer growth.
对五种肉源凝固酶阴性葡萄球菌进行了体外产3-甲基-1-丁醇的筛选。松鼠葡萄球菌alphaSG2尽管生长不佳，但3-甲基-1-丁醇的产量最高。对于通常用于香肠发酵剂培养的葡萄球菌种类，木糖葡萄球菌3PA6产生的3-甲基-1-丁醇比肉葡萄球菌833更多。采用数学建模将木糖葡萄球菌3PA6和肉葡萄球菌833在肉模拟培养基中产3-甲基-1-丁醇的动力学与细菌生长及环境因素（即温度和pH）联系起来。木糖葡萄球菌3PA6的3-甲基-1-丁醇比生产率比肉葡萄球菌833高约十倍，这表明每单位生物量的生产率更高。这就解释了尽管木糖葡萄球菌3PA6生长较差，但在含有该菌的培养基中3-甲基-1-丁醇浓度更高的原因。
