Moodley Arshnee, Guardabassi Luca
Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, Frederiksberg C 1870, Denmark.
Vet Microbiol. 2009 Jun 12;137(3-4):397-401. doi: 10.1016/j.vetmic.2009.01.034. Epub 2009 Jan 30.
A cross-sectional study was carried out to investigate species distribution and clonality of methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolated from the nasal cavity of 39 horses and 32 veterinary or caretaker staff, and from 76 environmental surfaces at three equine facilities in Denmark. MRCoNS obtained by selective isolation were characterised by 16S rDNA sequencing, PCR detection of mecA, and pulsed-field gel electrophoresis (PFGE). MRCoNS were found in 32 (82%) horses, 20 (63%) humans and 50 (66%) environmental samples. The most common species was S. vitulinus (n=56), followed by S. sciuri (n=25), S. haemolyticus (n=14). Undistinguishable PFGE patterns were observed in MRCoNS of equine, human and environmental origin. A S. vitulinus clone and a S. haemolyticus clone were isolated from multiple horses, staff members and environmental sites within a farm and a veterinary hospital, respectively. The results indicated that methicillin-resistant strains of these two species can be shared by and possibly exchanged between horses and personnel, either directly or through contaminated environments.
开展了一项横断面研究，以调查从丹麦三个马匹设施的39匹马、32名兽医或饲养员的鼻腔以及76个环境表面分离出的耐甲氧西林凝固酶阴性葡萄球菌（MRCoNS）的物种分布和克隆性。通过选择性分离获得的MRCoNS通过16S rDNA测序、mecA的PCR检测和脉冲场凝胶电泳（PFGE）进行表征。在32匹（82%）马、20名（63%）人以及50个（66%）环境样本中发现了MRCoNS。最常见的物种是维氏葡萄球菌（n = 56），其次是松鼠葡萄球菌（n = 25）、溶血葡萄球菌（n = 14）。在源自马、人和环境的MRCoNS中观察到了无法区分的PFGE模式。分别从一个农场和一家兽医医院内的多匹马、工作人员和环境地点分离出了一个维氏葡萄球菌克隆和一个溶血葡萄球菌克隆。结果表明，这两个物种的耐甲氧西林菌株可以在马和人员之间直接或通过受污染的环境共享并可能交换。
