Fraczkowska Kaja, Zak-Bochenek Agnieszka, Siwinska Natalia, Rypula Krzysztof, Ploneczka-Janeczko Katarzyna
Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Pathophysiology and Veterinary Preventive Medicine, Wrocław University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
Animals (Basel). 2022 Mar 17;12(6):756. doi: 10.3390/ani12060756.
From a clinical point of view, knowledge of the commensal microbial flora of the conjunctival sac in healthy individuals proves to be of great importance. The aim of this study was to assess the presence and the composition of normal ocular microflora of healthy donkeys. Fourteen clinically healthy donkeys () participated in the study. After prior ophthalmological examination, which showed no abnormalities, a conjunctival swab was taken from each donkey from the right and left eye. Species-specific identification was based on a morphological assessment of bacterial colonies stained with the Gram technique, as well as on biochemical properties and the disk-diffusion method. Around 82% of samples were positive for bacteria cultivation; was the most prevalently detected species, followed by . In conclusion, our study made it possible to determine the commensal flora of the conjunctival sac in donkeys. The obtained results also showed discrepancies in the composition of the conjunctival sac flora of donkeys and horses, despite the geographical proximity of performed studies. Knowledge of the commensal conjunctival flora of donkeys is of great clinical importance due to their greater exposure to corneal damage and infections than horses.
从临床角度来看，了解健康个体结膜囊的共生微生物群被证明非常重要。本研究的目的是评估健康驴正常眼微生物群的存在情况和组成。十四头临床健康的驴参与了该研究。在先前的眼科检查未发现异常后，从每头驴的右眼和左眼采集结膜拭子。物种特异性鉴定基于革兰氏染色细菌菌落的形态学评估，以及生化特性和纸片扩散法。约82%的样本细菌培养呈阳性； 是最常检测到的物种，其次是 。总之，我们的研究使得确定驴结膜囊的共生菌群成为可能。尽管所进行研究的地理位置相近，但所得结果也显示了驴和马结膜囊菌群组成的差异。由于驴比马更容易受到角膜损伤和感染，了解驴结膜共生菌群具有重要的临床意义。
