Engelsman Anton F, Krom Bastiaan P, Busscher Henk J, van Dam Gooitzen M, Ploeg Rutger J, van der Mei Henny C
Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, The Netherlands.
Acta Biomater. 2009 Jul;5(6):1905-10. doi: 10.1016/j.actbio.2009.01.041. Epub 2009 Feb 5.
Infection of surgical meshes used in abdominal wall reconstructions often leads to removal of the implant and increases patient morbidity due to repetitive operations and hospital administrations. Treatment with antibiotics is ineffective due to the biofilm mode of growth of the infecting bacteria and bears the risk of inducing antibiotic resistance. Hence there is a need for alternative methods to prevent and treat mesh infection. Nitric oxide (NO)-releasing coatings have been demonstrated to possess bactericidal properties in vitro. It is the aim of this study to assess possible benefits of a low concentration NO-releasing carbon-based coating on monofilament polypropylene meshes with respect to infection control in vitro and in vivo. When applied on surgical meshes, NO-releasing coatings showed significant bactericidal effect on in vitro biofilms of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and CNS. However, using bioluminescent in vivo imaging, no beneficial effects of this NO-releasing coating on subcutaneously implanted surgical meshes in mice could be observed.
用于腹壁重建的外科补片感染通常会导致植入物取出，并且由于重复手术和住院治疗增加了患者的发病率。由于感染细菌的生物膜生长模式，使用抗生素治疗无效，且有诱导抗生素耐药性的风险。因此，需要替代方法来预防和治疗补片感染。已证明释放一氧化氮（NO）的涂层在体外具有杀菌特性。本研究的目的是评估低浓度释放NO的碳基涂层在单丝聚丙烯补片上对体外和体内感染控制的潜在益处。当应用于外科补片时，释放NO的涂层对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和凝固酶阴性葡萄球菌的体外生物膜显示出显著的杀菌作用。然而，使用生物发光体内成像，未观察到这种释放NO的涂层对小鼠皮下植入的外科补片有有益作用。
