Research, ICTP-CSIC, Biomaterials, Juan de la Cierva 3, Madrid 28006, Spain.
Acta Biomater. 2013 Apr;9(4):6006-18. doi: 10.1016/j.actbio.2012.12.012. Epub 2012 Dec 20.
The application of bioactive meshes in abdominal surgery for the repair of hernias is an increasing clinical activity in a wide sector of the population. The main secondary effect is the appearance of infections from bacteria, specifically Staphylococcus aureus and S. epidermidis. This paper describes the development and application of low-density polypropylene meshes coated with a biocompatible and resorbable polymer as a controlled release system of the antibiotic vancomycin. The polymeric coating (a non-cross-linked copolymer of 2-hydroxyethyl methacrylate and 2-acrylamido-2-methylpropanesulfonic acid) has a thickness of 14-15μm and contains 0.32mgcm(-2) of the antibiotic vancomycin. The in vitro experiments demonstrate the excellent inhibitory character of the coated meshes loaded with the antibiotic, following the standard protocol of inhibition of halo in agar diffusion test. This inhibitory effect is maintained for a relatively long period (at least 14days) with a low concentration of antibiotic. The acrylic polymer system regulates the release of the antibiotic with a rate of 24μgh(-1), due to its slow dissolution in the medium. Experiments in vivo, based on the implantation of coated meshes, demonstrate that the system controls the infection in the animal (rabbits) for at least 30days. The concentration of antibiotic in the blood stream of the rabbits was below the detection limit of the analytical technique (<1-2μgml(-1)), which demonstrates that the antibiotic is released in the local area of the implant and remains concentrated at the implantation site, without diffusion to the blood stream. The systems can be applied to other medical devices and implants for the application of new-generation antibiotics in a controlled release and targeted applications.
生物活性网片在腹部手术中修复疝的应用在很大一部分人群中是一种日益增多的临床活动。主要的次要影响是细菌感染的出现，特别是金黄色葡萄球菌和表皮葡萄球菌。本文介绍了一种低密度聚丙烯网片的开发和应用，该网片涂有生物相容性和可吸收的聚合物，作为万古霉素抗生素的控释系统。聚合涂层（甲基丙烯酸羟乙酯和 2-丙烯酰胺-2-甲基丙磺酸的非交联共聚物）的厚度为 14-15μm，含有 0.32mgcm(-2)的抗生素万古霉素。体外实验表明，包被的网片在琼脂扩散试验的标准抑制环抑制实验中表现出良好的抑制特性。这种抑制作用在含有抗生素的涂层网片中维持相对较长的时间（至少 14 天），抗生素浓度较低。由于其在介质中的缓慢溶解，丙烯酸聚合物系统以 24μgh(-1)的速率调节抗生素的释放。体内实验基于涂层网片的植入，证明该系统至少可以在 30 天内控制动物（兔）的感染。兔血液中的抗生素浓度低于分析技术的检测限（<1-2μgml(-1))，这表明抗生素在植入物的局部区域释放，并保持在植入部位浓缩，而不扩散到血流中。该系统可应用于其他医疗设备和植入物，以在控释和靶向应用中应用新一代抗生素。
