Kimura Junko, Ono Tomoyuki, Sakamoto Kazuho, Ito Emi, Watanabe Shinya, Maeda Sachiko, Shikama Yayoi, Yatabe Midori Sasaki, Matsuoka Isao
Department of Pharmacology, Fukushima Medical University, School of Medicine, Japan.
Biol Pharm Bull. 2009 Mar;32(3):325-31. doi: 10.1248/bpb.32.325.
Here we reviewed our recent work on the chronic effects of nicotine on the Na+ -Ca2+ exchanger (NCX) gene and protein expressions in various organs of rats treated with nicotine in the drinking water for 4-12 weeks. Microarray analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) did not detect significant changes in NCX mRNA expression in cerebral cortex, hippocampus, heart and skeletal muscle. However, NCX1 protein was up-regulated by nicotine in cerebral cortex and hippocampus, but was down-regulated in the heart. NCX2 protein was up-regulated by nicotine in hippocampus. We suggest that although mRNA change was insignificant, NCX protein expression was altered by chronic nicotine administration in brain and heart in rats. We also reviewed our work on modulators of NCX gene expression and function in cardiac myocytes.
在此，我们回顾了我们最近的工作，该工作研究了尼古丁对通过饮用含尼古丁的水进行4至12周处理的大鼠各器官中钠钙交换体（NCX）基因和蛋白表达的慢性影响。微阵列分析和逆转录聚合酶链反应（RT-PCR）未检测到大脑皮层、海马体、心脏和骨骼肌中NCX mRNA表达的显著变化。然而，尼古丁使大脑皮层和海马体中的NCX1蛋白上调，但使心脏中的NCX1蛋白下调。尼古丁使海马体中的NCX2蛋白上调。我们认为，尽管mRNA变化不显著，但慢性给予尼古丁会改变大鼠脑和心脏中NCX蛋白的表达。我们还回顾了我们关于心肌细胞中NCX基因表达和功能调节剂的工作。
