Kühn Jonas, Montfort Frédéric, Colomb Tristan, Rappaz Benjamin, Moratal Corinne, Pavillon Nicolas, Marquet Pierre, Depeursinge Christian
Institute of Imaging and Applied Optics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Opt Lett. 2009 Mar 1;34(5):653-5. doi: 10.1364/ol.34.000653.
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485-670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6-1 microm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
我们展示了一种通过多波长数字全息显微镜实现具有亚微米轴向分辨率的无机械扫描层析成像方法的初步结果。通过顺序采集反射全息图并对485 - 670纳米范围内空间频率等间隔的20个波前进行求和，我们能够在生物介质中以0.6 - 1微米的轴向分辨率进行逐切片的层析重建。该方法应用于红细胞研究，以三维方式获取细胞膜轮廓。
