Thiyagarajan B, Valivittan K
Department of Zoology, Presidency College, Chennai, Tamilnadu, India.
J Assist Reprod Genet. 2009 Apr;26(4):217-25. doi: 10.1007/s10815-009-9302-1. Epub 2009 Feb 28.
Oxidative stress has been implicated in the etiology of defective embryo development. Vitamin E is an effective lipid-soluble antioxidant, protecting cell membranes from peroxidative damage. In this context, this study was undertaken to find if supplementation of vitamin E in culture medium could ameliorate the developmental competence of preimplantation buffalo embryos.
Vitamin E was supplemented in maturation/embryo culture medium at concentrations of 0, 50, 100, 200 and 400 microM. The developmental competence of buffalo embryos was assessed by observing the cleavage, morulae, blastocyst rate, total cell count and comet assay.
Vitamin E had no significant effect in maturation medium. Vitamin E in embryo culture medium under 5% O(2) significantly reduced blastocyst formation in the 400 microM supplemented group. Culture under 20% O(2) enhanced the frequency of blastocyst formation, total cell count and significantly reduced comet tail in the 100 microM supplemented group (P < 0.001) when compared with the control. Vitamin E in ECM for the first 72 h of culture period enhanced blastocyst rate and total cell number in the 100 microM group (P < 0.001) when compared with the control.
Our results demonstrate that the addition of Vitamin E may enhance the developmental competence of buffalo embryos in vitro by protecting them from oxidative stress.
氧化应激与胚胎发育缺陷的病因有关。维生素E是一种有效的脂溶性抗氧化剂，可保护细胞膜免受过氧化损伤。在此背景下，本研究旨在探讨在培养基中添加维生素E是否能改善水牛植入前胚胎的发育能力。
在成熟/胚胎培养基中添加浓度为0、50、100、200和400微摩尔的维生素E。通过观察卵裂、桑葚胚、囊胚率、总细胞数和彗星试验来评估水牛胚胎的发育能力。
维生素E在成熟培养基中无显著作用。在5%氧气条件下，胚胎培养基中添加400微摩尔维生素E显著降低了囊胚形成率。与对照组相比，在20%氧气条件下培养时，添加100微摩尔维生素E的组囊胚形成频率、总细胞数增加，彗星尾显著减少（P<0.001）。在培养的前72小时，在胚胎培养基中添加维生素E，与对照组相比，100微摩尔组的囊胚率和总细胞数增加（P<0.001）。
我们的结果表明，添加维生素E可能通过保护水牛胚胎免受氧化应激来提高其体外发育能力。
