中间球海胆SpRunt-1基因PCR-SSCP多态性分析及其与生长性状的相关性初步研究

Preliminary study on polymorphism analysis of SpRunt-1 gene by PCR-SSCP in Strongylocentrotus intermedius and its association with growth traits.

作者信息

Wang Xiuli, Qiu Xuemei, Meng Xiangying, Yang Lei

机构信息

College of Life Sciences and Biotechnology, Dalian Fisheries University, Dalian, China.

出版信息

Mol Biol Rep. 2010 Jan;37(1):411-5. doi: 10.1007/s11033-009-9481-y. Epub 2009 Feb 28.

DOI:10.1007/s11033-009-9481-y

PMID:19253002

Abstract

In current study, the SpRunt-1 gene was screened for the polymorphisms using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods in Strongylocentrotus intermedius (S. intermedius). Three polymorphisms were found with two site mutations, G833A and C1505T mutation, and an insertion mutation (GT) between 931 nt and 932 nt. These two site mutations are synonymous mutations and the insertion mutation is frame shift mutation. One way ANOVA analysis of correlation between the single nucleotide polymorphisms (SNPs) and growth traits showed that the gonad weight of AA genotype (G at 833 nt) was significantly higher than that of BB genotype (A at 833 nt) (P = 0.029). The body weight of the CC genotype (C at 1505 nt) was significantly higher than that of DD genotype (T at 1505 nt) (P < 0.01) and the shell height of CD genotype was significantly higher than that of DD genotype (P = 0.032). These results provided the evidence that S. intermedius SpRunt-1 gene could be selected as a candidate gene for the growth traits.

摘要

在当前研究中，运用聚合酶链反应-单链构象多态性（PCR-SSCP）和DNA测序方法，对中间球海胆（Strongylocentrotus intermedius）的SpRunt-1基因多态性进行筛选。发现了三个多态性位点，包括两个位点突变，即G833A和C1505T突变，以及一个位于931 nt和932 nt之间的插入突变（GT）。这两个位点突变是同义突变，插入突变是移码突变。对单核苷酸多态性（SNP）与生长性状之间的相关性进行单因素方差分析表明，AA基因型（833 nt处为G）的性腺重量显著高于BB基因型（833 nt处为A）（P = 0.029）。CC基因型（1505 nt处为C）的体重显著高于DD基因型（1505 nt处为T）（P < 0.01），CD基因型的壳高显著高于DD基因型（P = 0.032）。这些结果为中间球海胆SpRunt-1基因可作为生长性状的候选基因提供了证据。