Preza Dorita, Thiede Bernd, Olsen Ingar, Grinde Bjørn
Institute of Oral Biology, Faculty of Dentistry, University of Oslo, Norway.
Acta Odontol Scand. 2009;67(3):161-9. doi: 10.1080/00016350902751545.
Saliva is important for oral health, including the prevention of dental decay. The purpose of the present work was to indicate whether the parotid gland has altered function in the elderly, particularly in terms of proteins secreted, and whether its functional status could be associated with the presence of root caries.
Ductal parotid gland secretion was obtained from 51 individuals: 21 elderly with carious roots (Patients), 20 elderly (Controls), and 10 adults (Young) without root caries. Pooled aliquots were analyzed by liquid chromatography/tandem mass spectrometry to yield lists of major proteins present in the three groups.
Approximately 200 unique proteins were detected, of which 73 were identified repeatedly with high confidence and therefore included in the comparison. Some of the differences observed, when comparing Patients with respectively elderly Controls and the Young, resembled changes found in patients with Sjogren's syndrome, a condition associated with dental decay. Other changes involved proteins that are likely to impact on the oral microbiota, such as the absence of dermcidin and the presence of collagen in Patients. Cystatin S, a putative indicator of caries, was present at a higher level in Patients.
Parotid function tends to change upon aging, with possible consequences as to caries activity. However, analyses of individual samples revealed considerable variations in protein patterns.
唾液对口腔健康很重要，包括预防龋齿。本研究的目的是表明腮腺在老年人中功能是否发生改变，特别是在分泌的蛋白质方面，以及其功能状态是否与根龋的存在有关。
从51名个体获取腮腺导管分泌物：21名患有根龋的老年人（患者）、20名老年人（对照组）和10名无根龋的成年人（年轻人）。对混合的等分试样进行液相色谱/串联质谱分析，以得出三组中存在的主要蛋白质列表。
检测到约200种独特蛋白质，其中73种被高可信度重复鉴定，因此纳入比较。在将患者分别与老年对照组和年轻人进行比较时观察到的一些差异，类似于干燥综合征患者中发现的变化，干燥综合征是一种与龋齿相关的疾病。其他变化涉及可能影响口腔微生物群的蛋白质，例如患者中缺乏防御素且存在胶原蛋白。胱抑素S是一种假定的龋齿指标，在患者中水平较高。
腮腺功能随年龄增长往往会发生变化，可能对龋齿活动产生影响。然而，对个体样本的分析显示蛋白质模式存在相当大的差异。
