Department of Otorhinolaryngology, Medical University of Graz, Auenbruggerplatz 26, 8036, Graz, Austria.
Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
Sci Rep. 2017 Jul 19;7(1):5867. doi: 10.1038/s41598-017-06361-2.
This study aimed to undertake an initial, comparative analysis of the oral salivary microbiome of patients with oral and oropharyngeal squamous cell carcinoma versus healthy controls. This project, conceived as a pilot study, included 11 patients (1 female, 10 male, mean age 61.6 yrs., SD = 8.2 yrs.) and 11 healthy controls (1 female, 10 male, mean age 46.7 yrs., SD = 15.1 yrs.). Samples of saliva were analysed by high-throughput sequencing of the 16S rRNA gene using the MiSeq platform. Sequence data revealed microbial changes that may mirror disease progression and reflect clinical preconditions such as age, alcohol consumption, tumour size, lymph node status, smoking habit, and tumour HPV-positivity. Consequently, mapping microbial changes in patients with oral and oropharyngeal squamous cell carcinomas might improve our understanding of the pathobiology of the disease, and help in the design of novel diagnostic and treatment strategies.
本研究旨在对口腔和口咽鳞状细胞癌患者与健康对照者的口腔唾液微生物组进行初步比较分析。该项目作为一项初步研究，纳入了 11 名患者（1 名女性，10 名男性，平均年龄 61.6 岁，标准差 8.2 岁）和 11 名健康对照者（1 名女性，10 名男性，平均年龄 46.7 岁，标准差 15.1 岁）。使用 MiSeq 平台对 16S rRNA 基因进行高通量测序分析唾液样本。序列数据揭示了可能反映疾病进展的微生物变化，并反映了年龄、饮酒、肿瘤大小、淋巴结状态、吸烟习惯和肿瘤 HPV 阳性等临床前条件。因此，对口腔和口咽鳞状细胞癌患者的微生物变化进行映射可能有助于我们了解疾病的病理生物学，并有助于设计新的诊断和治疗策略。
