Flk1-myr::mCherry小鼠作为一种有用的报告基因，可用于表征眼部血管发育和疾病的多个方面。
The Flk1-myr::mCherry mouse as a useful reporter to characterize multiple aspects of ocular blood vessel development and disease.
作者信息
Poché Ross A, Larina Irina V, Scott Melissa L, Saik Jennifer E, West Jennifer L, Dickinson Mary E
机构信息
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA.
出版信息
Dev Dyn. 2009 Sep;238(9):2318-26. doi: 10.1002/dvdy.21886.
Abstract
The highly vascularized mouse eye is an excellent model system in which to elucidate the molecular genetic basis of blood vessel development and disease. However, the analysis of ocular vessel defects has traditionally been derived from fixed tissue, which fails to account for dynamic events such as blood flow and cell migration. To overcome the limitations of static analysis, tremendous advances in imaging technology and fluorescent protein reporter mouse lines now enable the direct visualization of developing cells in vivo. Here, we demonstrate that the Flk1-myr::mCherry transgenic mouse is an extremely useful live reporter with broad applicability to retinal, hyaloid, and choroid vascular research.
摘要
高度血管化的小鼠眼睛是一个极好的模型系统，可用于阐明血管发育和疾病的分子遗传基础。然而，传统上对眼部血管缺陷的分析来自固定组织，这无法解释诸如血流和细胞迁移等动态事件。为了克服静态分析的局限性，成像技术和荧光蛋白报告基因小鼠品系取得了巨大进展，现在能够在体内直接可视化发育中的细胞。在这里，我们证明Flk1-myr::mCherry转基因小鼠是一种极其有用的活体报告基因，广泛适用于视网膜、玻璃体和脉络膜血管研究。
相似文献
1
The Flk1-myr::mCherry mouse as a useful reporter to characterize multiple aspects of ocular blood vessel development and disease.Flk1-myr::mCherry小鼠作为一种有用的报告基因，可用于表征眼部血管发育和疾病的多个方面。
Dev Dyn. 2009 Sep;238(9):2318-26. doi: 10.1002/dvdy.21886.
2
A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.一种用于研究小鼠胚胎发育过程中血管重塑和心脏功能的膜相关mCherry荧光报告基因系。
Anat Rec (Hoboken). 2009 Mar;292(3):333-41. doi: 10.1002/ar.20821.
3
Limited versus total epithelial debridement ocular surface injury: Live fluorescence imaging of hemangiogenesis and lymphangiogenesis in Prox1-GFP/Flk1::Myr-mCherry mice.局限性与全层上皮清创性眼表损伤：Prox1-GFP/Flk1::Myr-mCherry小鼠血管生成和淋巴管生成的活体荧光成像
Biochim Biophys Acta. 2016 Oct;1860(10):2148-56. doi: 10.1016/j.bbagen.2016.05.027. Epub 2016 May 24.
4
Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice.在Prox1-GFP/Flk1::myr-mCherry小鼠中对血管和淋巴管生长进行同步体内成像。
FEBS J. 2015 Apr;282(8):1458-1467. doi: 10.1111/febs.13234. Epub 2015 Mar 6.
5
Macrophages engulf endothelial cell membrane particles preceding pupillary membrane capillary regression.巨噬细胞在瞳孔膜毛细血管消退之前吞噬内皮细胞膜颗粒。
Dev Biol. 2015 Jul 1;403(1):30-42. doi: 10.1016/j.ydbio.2015.03.017. Epub 2015 Apr 24.
6
A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature.一种用于将基因递送至血管生成性脉管系统的新型Flk1-TVA转基因小鼠模型。
Transgenic Res. 2008 Jun;17(3):403-15. doi: 10.1007/s11248-007-9156-1. Epub 2007 Nov 20.
7
Flk1-GFP BAC Tg mice: an animal model for the study of blood vessel development.Flk1-GFP BAC Tg 小鼠：一种用于血管发育研究的动物模型。
Exp Anim. 2010;59(5):615-22. doi: 10.1538/expanim.59.615.
8
Functional OCT angiography reveals early physiological dysfunction of hyaloid vasculature in developing mouse eye.功能光学相干断层扫描血管造影术揭示了发育中小鼠眼球中玻璃血管的早期生理功能障碍。
Exp Biol Med (Maywood). 2019 Jul;244(10):819-823. doi: 10.1177/1535370219850787. Epub 2019 May 24.
9
10
Evidence for novel fate of Flk1+ progenitor: contribution to muscle lineage.Flk1+祖细胞新命运的证据：对肌肉谱系的贡献。
Genesis. 2003 Mar;35(3):153-9. doi: 10.1002/gene.10175.
引用本文的文献
1
Longitudinal OCT and OCTA monitoring reveals accelerated regression of hyaloid vessels in retinal degeneration 10 (rd10) mice.纵向 OCT 和 OCTA 监测显示视网膜变性 10（rd10）小鼠的玻璃体液血管加速消退。
Sci Rep. 2019 Nov 13;9(1):16685. doi: 10.1038/s41598-019-53082-9.
2
Engineering the vasculature for islet transplantation.工程化血管用于胰岛移植。
Acta Biomater. 2019 Sep 1;95:131-151. doi: 10.1016/j.actbio.2019.05.051. Epub 2019 May 23.
3
Overview of the reporter genes and reporter mouse models.报告基因及报告基因小鼠模型概述。
Animal Model Exp Med. 2018 Apr 19;1(1):29-35. doi: 10.1002/ame2.12008. eCollection 2018 Mar.
4
Methods to label, image, and analyze the complex structural architectures of microvascular networks.标记、成像和分析微血管网络复杂结构体系的方法。
Microcirculation. 2019 Jul;26(5):e12520. doi: 10.1111/micc.12520. Epub 2019 Jan 17.
5
Modes of Antigen Presentation by Lymph Node Stromal Cells and Their Immunological Implications.淋巴结基质细胞的抗原呈递模式及其免疫学意义。
Front Immunol. 2015 Sep 8;6:446. doi: 10.3389/fimmu.2015.00446. eCollection 2015.
6
Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules.对巨噬细胞招募分子局部递送的反应中血管生成的改善
PLoS One. 2015 Jul 1;10(7):e0131643. doi: 10.1371/journal.pone.0131643. eCollection 2015.
7
Macrophages engulf endothelial cell membrane particles preceding pupillary membrane capillary regression.巨噬细胞在瞳孔膜毛细血管消退之前吞噬内皮细胞膜颗粒。
Dev Biol. 2015 Jul 1;403(1):30-42. doi: 10.1016/j.ydbio.2015.03.017. Epub 2015 Apr 24.
8
Simultaneous in vivo imaging of blood and lymphatic vessel growth in Prox1-GFP/Flk1::myr-mCherry mice.在Prox1-GFP/Flk1::myr-mCherry小鼠中对血管和淋巴管生长进行同步体内成像。
FEBS J. 2015 Apr;282(8):1458-1467. doi: 10.1111/febs.13234. Epub 2015 Mar 6.
9
Quantitative imaging of cell dynamics in mouse embryos using light-sheet microscopy.使用光片显微镜对小鼠胚胎中的细胞动力学进行定量成像。
Development. 2014 Nov;141(22):4406-14. doi: 10.1242/dev.111021. Epub 2014 Oct 24.
10
In vivo analysis of hyaloid vasculature morphogenesis in zebrafish: A role for the lens in maturation and maintenance of the hyaloid.斑马鱼透明血管系统形态发生的体内分析：晶状体在透明血管系统成熟和维持中的作用。
Dev Biol. 2014 Oct 15;394(2):327-39. doi: 10.1016/j.ydbio.2014.07.024. Epub 2014 Aug 13.
本文引用的文献
1
A membrane associated mCherry fluorescent reporter line for studying vascular remodeling and cardiac function during murine embryonic development.一种用于研究小鼠胚胎发育过程中血管重塑和心脏功能的膜相关mCherry荧光报告基因系。
Anat Rec (Hoboken). 2009 Mar;292(3):333-41. doi: 10.1002/ar.20821.
2
Vascular precursors in developing human retina.发育中的人类视网膜中的血管前体细胞。
Invest Ophthalmol Vis Sci. 2008 May;49(5):2178-92. doi: 10.1167/iovs.07-0632.
3
Noninvasive in vivo imaging of pancreatic islet cell biology.胰岛细胞生物学的无创体内成像
Nat Med. 2008 May;14(5):574-8. doi: 10.1038/nm1701. Epub 2008 Mar 7.
4
The mouse cornea micropocket angiogenesis assay.小鼠角膜微袋血管生成试验。
Nat Protoc. 2007;2(10):2545-50. doi: 10.1038/nprot.2007.368.
5
Maintaining transparency: a review of the developmental physiology and pathophysiology of two avascular tissues.保持透明：两种无血管组织的发育生理学和病理生理学综述
Semin Cell Dev Biol. 2008 Apr;19(2):125-33. doi: 10.1016/j.semcdb.2007.08.014. Epub 2007 Sep 1.
6
Corneal graft rejection.角膜移植排斥反应。
Surv Ophthalmol. 2007 Jul-Aug;52(4):375-96. doi: 10.1016/j.survophthal.2007.04.008.
7
Fibrosis and diseases of the eye.眼部纤维化及相关疾病
J Clin Invest. 2007 Mar;117(3):576-86. doi: 10.1172/JCI31030.
8
Development of the retinal vasculature.视网膜血管系统的发育。
Angiogenesis. 2007;10(2):77-88. doi: 10.1007/s10456-007-9065-1. Epub 2007 Feb 24.
9
The initial fetal human retinal vasculature develops by vasculogenesis.最初的人类胎儿视网膜血管系统通过血管生成发育而成。
Dev Dyn. 2006 Dec;235(12):3336-47. doi: 10.1002/dvdy.20988.
10
Corneal avascularity is due to soluble VEGF receptor-1.角膜无血管状态归因于可溶性血管内皮生长因子受体-1。
Nature. 2006 Oct 26;443(7114):993-7. doi: 10.1038/nature05249. Epub 2006 Oct 18.
Zotero 插件