Poché Ross A, Larina Irina V, Scott Melissa L, Saik Jennifer E, West Jennifer L, Dickinson Mary E
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA.
Dev Dyn. 2009 Sep;238(9):2318-26. doi: 10.1002/dvdy.21886.
The highly vascularized mouse eye is an excellent model system in which to elucidate the molecular genetic basis of blood vessel development and disease. However, the analysis of ocular vessel defects has traditionally been derived from fixed tissue, which fails to account for dynamic events such as blood flow and cell migration. To overcome the limitations of static analysis, tremendous advances in imaging technology and fluorescent protein reporter mouse lines now enable the direct visualization of developing cells in vivo. Here, we demonstrate that the Flk1-myr::mCherry transgenic mouse is an extremely useful live reporter with broad applicability to retinal, hyaloid, and choroid vascular research.
高度血管化的小鼠眼睛是一个极好的模型系统，可用于阐明血管发育和疾病的分子遗传基础。然而，传统上对眼部血管缺陷的分析来自固定组织，这无法解释诸如血流和细胞迁移等动态事件。为了克服静态分析的局限性，成像技术和荧光蛋白报告基因小鼠品系取得了巨大进展，现在能够在体内直接可视化发育中的细胞。在这里，我们证明Flk1-myr::mCherry转基因小鼠是一种极其有用的活体报告基因，广泛适用于视网膜、玻璃体和脉络膜血管研究。
