Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, 60607, USA.
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, 60612, USA.
Sci Rep. 2019 Nov 13;9(1):16685. doi: 10.1038/s41598-019-53082-9.
The hyaloid vascular system (HVS) is known to have an important role in eye development. However, physiological mechanisms of HVS regression and their correlation with developmental eye disorders remain unclear due to technical limitations of conventional ending point examination with fixed tissues. Here, we report comparative optical coherence tomography (OCT) and OCT angiography (OCTA) monitoring of HVS regression in wild-type and retinal degeneration 10 (rd10) mice. Longitudinal OCTA monitoring revealed accelerated regression of hyaloid vessels correlated with retinal degeneration in rd10. Quantitative OCT measurement disclosed significant distortions of both retinal thickness and the vitreous chamber in rd10 compared to WT mice. These OCT/OCTA observations confirmed the close relationship between HVS physiology and retinal neurovascular development. The distorted HVS regression might result from retinal hyperoxia or dopamine abnormality due to retinal remodeling in rd10 retina. By providing a noninvasive imaging platform for longitudinal monitoring of HVS regression, further OCT/OCTA study may lead to in-depth understanding of the physiological mechanisms of HVS regression in normal and diseased eyes, which is not only important for advanced study of the nature of the visual system but also may provide insights into the development of better treatment protocols of congenital eye disorders.
玻璃血管系统（HVS）在眼睛发育中具有重要作用。然而，由于传统的固定组织终点检查技术的限制，HVS 退化的生理机制及其与发育性眼病的相关性仍不清楚。在这里，我们报告了野生型和视网膜变性 10 型（rd10）小鼠玻璃血管系统（HVS）退化的比较光学相干断层扫描（OCT）和 OCT 血管造影（OCTA）监测。纵向 OCTA 监测显示，HVS 退化在 rd10 中与视网膜变性相关，呈加速趋势。与 WT 小鼠相比，定量 OCT 测量显示 rd10 中的视网膜厚度和玻璃体腔存在明显扭曲。这些 OCT/OCTA 观察结果证实了 HVS 生理学与视网膜神经血管发育之间的密切关系。rd10 视网膜中的视网膜过度氧化或多巴胺异常可能导致 HVS 退化的扭曲。通过提供 HVS 退化的纵向监测的非侵入性成像平台，进一步的 OCT/OCTA 研究可能深入了解正常和患病眼睛中 HVS 退化的生理机制，这不仅对视觉系统本质的深入研究很重要，还可能为先天性眼病的更好治疗方案的发展提供思路。
