[聚天冬酰胺-谷氨酸接枝的低分子量聚乙烯亚胺作为一种新型非病毒基因载体]
[Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector].
作者信息
Chen Dan, He Bin-Bin, Zhao Dan-Jun, Jiang Qi-Ying, Wang Zhong-Ren, Zhou Jun, Yu Hai, Wang Qing-Qing, Tang Gu-Ping
机构信息
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):31-8. doi: 10.3785/j.issn.1008-9292.2009.01.005.
OBJECTIVE
To develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone.
METHODS
alpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified.
RESULT
The polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines.
CONCLUSION
PHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.
目的
以聚天冬酰胺-谷氨酸和聚乙烯亚胺为骨架开发一种新型基因递送载体。
方法
合成α，β-聚（N-2-羟丙基）-D，L-天冬酰胺-谷氨酸（PHPAG），并接枝低分子量聚乙烯亚胺（PEI 1.8 kDa）以形成PHPAG-PEI 1800。对该聚合物进行化学和生物学表征。
结果
通过（1）H-NMR确认该聚合物，分子量约为1.2×10（4）。凝胶阻滞试验显示在N/P比为3.5时具有DNA结合能力。MTT试验表明该聚合物在COS-7和A293细胞系中无毒。体外试验表明它在B16和Hela细胞系中具有高转染效率。
结论
成功合成了PHPAG-PEI 1800，其可能是一种潜在的基因递送载体。
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