Voronov-Goldman Milana, Noach Ilit, Lamed Raphael, Shimon Linda J W, Borovok Ilya, Bayer Edward A, Frolow Felix
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Israel.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):275-8. doi: 10.1107/S1744309109002887. Epub 2009 Feb 26.
A cohesin-like module of 160 amino-acid residues from the hypothetical protein AF2375 of the noncellulolytic, hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus was cloned, expressed, purified, crystallized and subjected to X-ray structural study in order to compare its structure with those of cellulolytic cohesins. The crystals had cubic symmetry, with unit-cell parameters a = b = c = 101.75 A in space group P4(3)32, and diffracted to 1.82 A resolution. The asymmetric unit contained a single cohesin molecule. A model assembled from six cohesin structures (PDB entries 1anu, 1aoh, 1g1k, 1qzn, 1zv9 and 1tyj) of very low sequence identity to the cohesin-like module was used in molecular-replacement attempts, producing a marginal solution.
从非纤维素分解、嗜热、硫酸盐还原古菌嗜热栖热菌（Archaeoglobus fulgidus）的假定蛋白AF2375中选取了一个由160个氨基酸残基组成的类黏连蛋白模块进行克隆、表达、纯化、结晶，并进行X射线结构研究，以便将其结构与纤维素分解黏连蛋白的结构进行比较。晶体具有立方对称性，在空间群P4(3)32中，晶胞参数a = b = c = 101.75 Å，衍射分辨率达到1.82 Å。不对称单元包含一个单一的黏连蛋白分子。在分子置换尝试中，使用了由六个与类黏连蛋白模块序列同一性极低的黏连蛋白结构（PDB条目1anu、1aoh、1g1k、1qzn、1zv9和1tyj）组装而成的模型，得到了一个勉强可用的结果。
