Knop Katrin, Mingotaud Anne-Françoise, El-Akra Naram, Violleau Frédéric, Souchard Jean-Pierre
Université de Toulouse; UPS/CNRS, IMRCP, 118 route de Narbonne, F-31062, Toulouse Cedex 9, France.
Photochem Photobiol Sci. 2009 Mar;8(3):396-404. doi: 10.1039/b811248g. Epub 2009 Jan 21.
Incorporation of unaggregated monomeric molecules of pheophorbide(a) into micelles of poly(ethyleneoxide-b-epsilon-caprolactone) has been characterized by fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy and asymmetric flow field flow fractionation. It was shown that the method used leads to 20 nm micelles, corresponding to approximately 200 molecules of polymer and 4 molecules of monomeric pheophorbide(a) per nano-object which was able to generate (1)O(2) in the medium. They have been used thereafter as nanocarriers for photosensitizers in photodynamic therapy against cancer cells. The encapsulation of photosensitizer has been verified and in vitro tests on human cancerous cells have revealed a ca. 2-fold enhanced photocytotoxicity and cellular uptake compared to free pheophorbide(a).
通过荧光光谱、动态光散射、透射电子显微镜和不对称流场流分级法对脱镁叶绿酸（a）的非聚集单体分子掺入聚（环氧乙烷-b-ε-己内酯）胶束进行了表征。结果表明，所使用的方法可形成20 nm的胶束，每个纳米物体对应约200个聚合物分子和4个单体脱镁叶绿酸（a）分子，这些纳米物体能够在介质中产生单线态氧。此后，它们被用作光动力疗法中针对癌细胞的光敏剂纳米载体。已证实了光敏剂的包封，对人癌细胞的体外测试表明，与游离脱镁叶绿酸（a）相比，光细胞毒性和细胞摄取提高了约2倍。
