Baechler Benjamin J, Nita Florina, Jones Lon, Frestedt Joy L
Eniva Nutraceuticals, Blaine, MN 55434, USA.
Plant Foods Hum Nutr. 2009 Jun;64(2):81-5. doi: 10.1007/s11130-009-0107-2.
This study explored the DNA protective (anti-mutagenic) effects of an oral, liquid, multi-phytonutrient dietary supplement containing a proprietary blend of fruits, vegetables and aloe vera concentrated components in addition to a proprietary catechin complex from green tea (VIBE Cardiac & Life, Eniva Nutraceuticals, Anoka, MN; herein described as "VIBE"). This study tested the hypothesis that VIBE would reduce DNA damage in skin cells exposed to UVR. Human epidermal cells, from the cell line A431NS, were treated with 0% (control), 0.125%, 0.5%, 1% and 2% VIBE, and then exposed to 240 J/m(2) UVR. The amount of DNA damage was assessed using the COMET assay. At each concentration tested, a significantly smaller amount of DNA damage was measured by the COMET assay for the VIBE treated cells compared to the control cells exposed to UVR without VIBE. The dose response curves showed a maximal response at 0.5% VIBE with a threefold reduction in COMET tail density compared to the control samples without VIBE (p < 0.001). Additional research is warranted in human clinical trials to further explore the results of this study which demonstrated the DNA protective and anti-mutagenic effects of VIBE for human skin cells exposed to UVR-induced DNA damage.
本研究探讨了一种口服液体多种植物营养素膳食补充剂的DNA保护（抗诱变）作用，该补充剂除了含有一种来自绿茶的专利儿茶素复合物外，还含有水果、蔬菜和芦荟浓缩成分的专利混合物（VIBE心脏与生命，Eniva营养制品公司，明尼苏达州阿诺卡；本文称为“VIBE”）。本研究检验了VIBE可减少暴露于紫外线辐射（UVR）的皮肤细胞中DNA损伤这一假设。来自A431NS细胞系的人表皮细胞分别用0%（对照）、0.125%、0.5%、1%和2%的VIBE处理，然后暴露于240 J/m(2)的UVR下。使用彗星试验评估DNA损伤量。在每个测试浓度下，与未使用VIBE而暴露于UVR的对照细胞相比，彗星试验测得VIBE处理细胞的DNA损伤量显著更少。剂量反应曲线显示，在VIBE浓度为0.5%时出现最大反应，与未使用VIBE的对照样品相比，彗星尾密度降低了三倍（p < 0.001）。有必要在人体临床试验中进行进一步研究，以进一步探索本研究的结果，该结果证明了VIBE对暴露于UVR诱导的DNA损伤的人体皮肤细胞具有DNA保护和抗诱变作用。
