Yang Seong-Won, Lee Byung Rae, Koh Jae-Woong
Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea.
Korean J Ophthalmol. 2007 Dec;21(4):232-7. doi: 10.3341/kjo.2007.21.4.232.
To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells.
UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay.
The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
评估表没食子儿茶素没食子酸酯（EGCG）对培养的人视网膜色素上皮（RPE）细胞紫外线照射的保护作用。
用紫外线灯以3.3 mW/cm2的辐照度照射30秒产生紫外线。5分钟和1小时后，给予不同浓度的EGCG（0、5、10、15、25、50、100 μM）。使用计数室在显微镜下确定细胞计数，并通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）法评估细胞活性。
与未给药组相比，EGCG给药组紫外线照射后培养的人RPE细胞计数明显增加。MTT法测定显示，EGCG给药组紫外线照射后培养的人RPE细胞活性明显增加，并呈剂量依赖性增加。
EGCG给药增加了紫外线照射后培养的人视网膜色素上皮细胞的细胞计数和细胞活性；这表明EGCG对培养的人视网膜色素上皮细胞的紫外线损伤具有保护作用。
