Klaassen C H W
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Med Mycol. 2009;47 Suppl 1:S27-33. doi: 10.1080/13693780802382244. Epub 2009 Feb 28.
In recent years, there has been a clear and growing tendency to use exact typing methods for discrimination between microbial isolates. Exact typing methods that yield an unambiguous typing result offer a number of advantages over conventional methods in the generation of typing data that is reproducible, portable and exchangeable. Two such methods are multi-locus sequence typing (MLST) and microsatellite-based typing. Here I will discuss the basic principles of both methods and compare them from a practical and performance point of view with respect to typing Aspergillus fumigatus isolates. Microsatellites offer the best available typing option by outperforming MLST in terms of speed, throughput, costs and discriminatory power. This latter advantage of microsatellites is a direct consequence of their inherent instability. This (in)stability of individual microsatellite markers and alleles should be taken into account in the interpretation of microsatellite-based typing data.
近年来，使用精确分型方法鉴别微生物分离株的趋势愈发明显且不断增长。能产生明确分型结果的精确分型方法在生成可重复、便携且可交换的分型数据方面，比传统方法具有诸多优势。多基因座序列分型（MLST）和基于微卫星的分型就是这样的两种方法。在此，我将讨论这两种方法的基本原理，并从实际操作和性能角度，就烟曲霉分离株的分型对它们进行比较。微卫星在速度、通量、成本和鉴别力方面优于MLST，提供了最佳的可用分型选择。微卫星的这一优势是其固有不稳定性的直接结果。在解释基于微卫星的分型数据时，应考虑单个微卫星标记和等位基因的这种（不）稳定性。
