Department of Dermatology, the Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Med Mycol. 2010 Feb;48(1):201-6. doi: 10.3109/13693780902785320.
Chromoblastomycosis is a chronic, cutaneous and subcutaneous infection characterized by verrucose lesions. It is primarily caused by Fonsecaea monophora in southern China and responds poorly to available therapies. In order to investigate the pathogenicity of Fonsecaea monophora, we established a chronic chromoblastomycosis animal model in Wistar rats. Suspensions of 2x10(6) cfu conidia and hyphal fragments were intracutaneous (ic) and subcutaneous (sc) on either side of the backs of the rats. Small nodules were formed at the inoculation sites within the first week after inoculation. By the second week, the nodules had enlarged and become soft, and pus could be aspirated from them. In the fourth week, the nodules in the ic inoculated group had ulcerated and sclerotic bodies were observed in smears prepared from all animals. In the 3rd month after inoculation, the nodules at the sites of the ic inoculation had become flat, with a thin black crust on the surface. For the ic inoculated group, sequential biopsy studies revealed extensive necrosis with neutrophil infiltration and sclerotic bodies and some debris of fungi in the 1st month, sclerotic bodies inside multinucleated giant cells by the 2nd month and widespread granulomatous inflammations in the 3rd month.
着色芽生菌病是一种慢性皮肤和皮下感染，其特征为疣状病变。它主要由中国南方的单端孢霉引起，对现有疗法反应不佳。为了研究单端孢霉的致病性，我们在 Wistar 大鼠中建立了慢性着色芽生菌病动物模型。在大鼠背部两侧的皮内（ic）和皮下（sc）接种 2x10(6)cfu 分生孢子和菌丝片段的悬浮液。接种后第一周内接种部位形成小结节。到第二周，结节已经增大并且变软，可以从其中抽吸脓液。第四周时，ic 接种组的结节已经溃疡，并且从所有动物制备的涂片上观察到硬化体。接种后 3 个月，ic 接种部位的结节变得平坦，表面有一层薄薄的黑色结痂。对于 ic 接种组，连续活检研究显示第 1 个月广泛的坏死伴中性粒细胞浸润和硬化体，以及一些真菌碎片，第 2 个月在多核巨细胞内有硬化体，第 3 个月广泛的肉芽肿性炎症。
