Tanamai Wendy, Chen Cynthia, Siavoshi Sara, Cerussi Albert, Hsiang David, Butler John, Tromberg Bruce
University of California, Irvine, Beckman Laser Institute, Laser Medical and Microbeam Program, 1002 Health Sciences Road East, Irvine, California 92612, USA.
J Biomed Opt. 2009 Jan-Feb;14(1):014024. doi: 10.1117/1.3028012.
Diffuse optical spectroscopy (DOS) has been used to monitor and predict the effects of neoadjuvant (i.e., presurgical) chemotherapy in breast cancer patients in several pilot studies. Because patients with suspected breast cancers undergo biopsy prior to treatment, it is important to understand how biopsy trauma influences DOS measurements in the breast. The goal of this study was to measure the effects of a standard core breast biopsy on DOS measurements of tissue deoxyhemoglobin, hemoglobin, water, and bulk lipid concentrations. We serially monitored postbiopsy effects in the breast tissue in a single subject (31-year-old premenopausal female) with a 37x18x20 mm fibroadenoma. A baseline measurement and eight weekly postbiopsy measurements were taken with a handheld DOS imaging instrument. Our instrument used frequency domain photon migration combined with broadband steady-state spectroscopy to characterize tissues via quantitative measurements of tissue absorption and reduced scattering coefficients from 650 to 1000 nm. The concentrations of significant near-infrared (NIR) absorbers were mapped within a 50 cm(2) area over the biopsied region. A 2-D image of a contrast function called the tissue optical index (TOI=deoxyhemoglobinxwaterbulk lipid) was generated and revealed that a minimum of 14 days postbiopsy was required to return TOI levels in the biopsied area to their prebiopsy levels. Changes in the TOI images of the fibroadenoma also reflected the progression of the patient's menstrual cycle. DOS could therefore be useful in evaluating both wound-healing response and the effects of hormone and hormonal therapies in vivo.
在多项初步研究中，漫射光光谱法（DOS）已被用于监测和预测新辅助（即术前）化疗对乳腺癌患者的疗效。由于疑似乳腺癌患者在治疗前需接受活检，因此了解活检创伤如何影响乳房的DOS测量结果很重要。本研究的目的是测量标准的乳腺芯针活检对组织中脱氧血红蛋白、血红蛋白、水和总脂质浓度的DOS测量结果的影响。我们对一名患有37x18x20 mm纤维腺瘤的31岁绝经前女性的乳腺组织活检后的效果进行了连续监测。使用手持式DOS成像仪进行了一次基线测量和八次活检后的每周测量。我们的仪器采用频域光子迁移结合宽带稳态光谱法，通过对650至1000 nm的组织吸收和约化散射系数进行定量测量来表征组织。在活检区域上方50 cm²的区域内绘制了重要近红外（NIR）吸收剂的浓度图。生成了一个名为组织光学指数（TOI = 脱氧血红蛋白×水×总脂质）的对比函数的二维图像，结果显示活检后至少需要14天才能使活检区域的TOI水平恢复到活检前水平。纤维腺瘤的TOI图像变化也反映了患者月经周期的进展。因此，DOS在评估体内伤口愈合反应以及激素和激素疗法的效果方面可能会很有用。
