Ueda Shigeto, Nakamiya Noriko, Matsuura Kazuo, Shigekawa Takashi, Sano Hiroshi, Hirokawa Eiko, Shimada Hiroko, Suzuki Hiroaki, Oda Motoki, Yamashita Yutaka, Kishino Osamu, Kuji Ichiei, Osaki Akihiko, Saeki Toshiaki
Department of Breast Oncology, International Medical Center, Saitama Medical University, Hidaka City 350-1298, Saitama, Japan.
BMC Cancer. 2013 Oct 31;13:514. doi: 10.1186/1471-2407-13-514.
Near-infrared optical imaging targeting the intrinsic contrast of tissue hemoglobin has emerged as a promising approach for visualization of vascularity in cancer research. We evaluated the usefulness of diffuse optical spectroscopy using time-resolved spectroscopic (TRS) measurements for functional imaging of primary breast cancer.
Fifty-five consecutive TNM stage I/II patients with histologically proven invasive ductal carcinoma and operable breast tumors (<5 cm) who underwent TRS measurements were enrolled. Thirty (54.5%) patients underwent 18F-fluoro-deoxy-glucose (FDG) positron emission tomography with measurement of maximum tumor uptake. TRS was used to obtain oxyhemoglobin, deoxyhemoglobin, and total hemoglobin (tHb) levels from the lesions, surrounding normal tissue, and contralateral normal tissue. Lesions with tHb levels 20% higher than those present in normal tissue were defined as "hotspots," while others were considered "uniform." The findings in either tumor type were compared with clinicopathological factors.
"Hotspot" tumors were significantly larger (P= 0.002) and exhibited significantly more advanced TNM stage (P=0.01), higher mitotic counts (P=0.01) and higher levels of FDG uptake (P=0.0004) compared with "uniform" tumors; however, other pathological variables were not significantly different between the two groups.
Optical imaging for determination of tHb levels allowed for measurement of tumor vascularity as a function of proliferation and glucose metabolism, which may be useful for prediction of patient prognosis and potential response to treatment.
针对组织血红蛋白固有对比度的近红外光学成像已成为癌症研究中血管可视化的一种有前景的方法。我们评估了使用时间分辨光谱（TRS）测量的漫射光学光谱对原发性乳腺癌进行功能成像的有用性。
连续纳入55例经组织学证实为浸润性导管癌且可手术切除的乳腺肿瘤（<5 cm）的TNM I/II期患者，这些患者均接受了TRS测量。30例（54.5%）患者接受了18F-氟脱氧葡萄糖（FDG）正电子发射断层扫描，并测量了最大肿瘤摄取量。TRS用于获取病变、周围正常组织和对侧正常组织中的氧合血红蛋白、脱氧血红蛋白和总血红蛋白（tHb）水平。tHb水平比正常组织高20%的病变被定义为“热点”，其他则被视为“均匀”。将两种肿瘤类型的结果与临床病理因素进行比较。
与“均匀”肿瘤相比，“热点”肿瘤明显更大（P = 0.002），TNM分期明显更高（P = 0.01），有丝分裂计数更高（P = 0.01），FDG摄取水平更高（P = 0.0004）；然而，两组之间的其他病理变量没有显著差异。
用于测定tHb水平的光学成像能够测量作为增殖和葡萄糖代谢函数的肿瘤血管生成，这可能有助于预测患者预后和潜在的治疗反应。
