Timmers Jaap, Vernhettes Samantha, Desprez Thierry, Vincken Jean-Paul, Visser Richard G F, Trindade Luisa M
CRC-Wageningen, Wageningen University and Research Centre, 6703 HD Wageningen, The Netherlands.
FEBS Lett. 2009 Mar 18;583(6):978-82. doi: 10.1016/j.febslet.2009.02.035. Epub 2009 Mar 1.
It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins.
关于次生纤维素合成酶（CESA）蛋白如何在玫瑰花结结构中组织，目前尚未见报道。采用基于膜的酵母双杂交（MbYTH）方法分析拟南芥次生细胞壁合成中涉及的CESA蛋白之间的相互作用，并通过双分子荧光互补（BiFC）分析在植物体内进行了验证。结果表明，虽然所有CESA蛋白都能相互作用，但只有CESA4能够形成同型二聚体。提出了一个次生玫瑰花结结构的模型。结果证明，RING基序对于CESA蛋白之间的相互作用并非必不可少。
