印度尼西亚献血者中的丙型肝炎病毒基因型及相关肝病
Hepatitis C virus genotype in blood donors and associated liver disease in Indonesia.
作者信息
Utama Andi, Budiarto Bugi Ratno, Monasari Dewi, Octavia Theresia Imelda, Chandra Ivan Stevanus, Gani Rino Alvani, Hasan Irsan, Sanityoso Andri, Miskad Upik Anderiani, Yusuf Irawan, Lesmana Laurentius Adrianus, Sulaiman Ali, Tai Susan
机构信息
Molecular Epidemiology Division, Mochtar Riady Institute for Nanotechnology, Lippo Karawaci, Tangerang, Indonesia.
出版信息
Intervirology. 2008;51(6):410-6. doi: 10.1159/000205515. Epub 2009 Mar 4.
OBJECTIVE
The aim of this study was to investigate the distribution of hepatitis C virus (HCV) genotype and the possible association between genotype and HCV-associated liver disease in Indonesia.
METHODS
32 anti-HCV-positive asymptomatic carriers (AC), 55 chronic hepatitis (CH), 41 liver cirrhosis (LC), and 35 hepatocellular carcinoma (HCC) patients were included in this study. HCV genotyping was performed by phylogenetic analysis of the NS5B and 5'-UTR regions.
RESULTS
The HCV subtype 1b (36.5%), based on NS5B region, was the most prevalent, followed by subtypes 3k (15.4%), 2a (14.4%), 1a (12.5%) and 1c (12.5%), and 2e (4.8%). Subtypes 2f, 3a, 3b, and 4a were also found in some of the samples. HCV subtypes 3k (40.0%) and 1a (35.0%) were the two major subtypes in AC. HCV subtype 1b was not found in AC, but it was common in CH (31.3%), LC (50.0%), and HCC (57.1%).
CONCLUSION
HCV subtype 1b was prevalent in samples of HCV-associated liver disease patients, including CH, LC and HCC. The percentage of subtype 1b was increased with the disease severity (AC < CH < LC < HCC).
目的
本研究旨在调查印度尼西亚丙型肝炎病毒（HCV）基因型的分布情况以及基因型与HCV相关肝病之间的可能关联。
方法
本研究纳入了32例抗HCV阳性无症状携带者（AC）、55例慢性肝炎（CH）患者、41例肝硬化（LC）患者和35例肝细胞癌（HCC）患者。通过对NS5B和5'-UTR区域进行系统发育分析来进行HCV基因分型。
结果
基于NS5B区域，HCV 1b型（36.5%）最为常见，其次是3k型（15.4%）、2a型（14.4%）、1a型（12.5%）、1c型（12.5%）和2e型（4.8%）。在一些样本中也发现了2f型、3a型、3b型和4a型。HCV 3k型（40.0%）和1a型（35.0%）是AC中的两种主要亚型。AC中未发现HCV 1b型，但在CH（31.3%）、LC（50.0%）和HCC（57.1%）中较为常见。
结论
HCV 1b型在HCV相关肝病患者的样本中普遍存在，包括CH、LC和HCC。1b型的比例随着疾病严重程度增加（AC < CH < LC < HCC）。
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