Hepatology Laboratory, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
J Clin Virol. 2013 Aug;57(4):311-7. doi: 10.1016/j.jcv.2013.04.013. Epub 2013 May 21.
We have recently determined HCV isolates among volunteer blood donors and IDUs in southern China and revealed the genotype distribution patterns not only different between the two studied cohorts but also from what we have sampled in 2002. A changed pattern could have also occurred among patients with liver disease.
Both E1 and NS5B sequences of HCV were characterized among 393 patients with liver disease followed by phylogenetic analysis.
Six HCV genotypes, 12 subtypes (1b: 65.9%, 6a: 17.1%, 2a: 7.4%, 3a: 3.6%, 3b: in 3.3%, 6e: 0.76%, and 1a, 1c, 2b, 2f, 4d, and 5a each 0.25%), and two novel genotype 6 variants were classified, showing the greatest complexity of HCV hitherto found in China. Although the predominance of 1b followed by 6a is largely consistent with what we have sampled in 2002, the identification of single isolates of 1c, 2f, 4d, 5a, and two novel HCV-6 variants were first reported. Excluding 4d from a European visitor, all the others were from Chinese patients. Since the 6a proportion (17.1%, 67/393) was unexpectedly lower than what we have recently detected among blood donors (34.8%, 82/236) and IDUs (51.5%, 70/136), further statistical analyses were conducted. Comparison of the mean ages showed that among the 393 patients, those infected with 1b were significantly (6.7 years) older than those with 6a, while the 393 patients as a whole were significantly older than the 236 blood donors (8.4 years) and 136 IDUs (12.6 years) we have recently reported. Explanations are that younger individuals had higher proportions of 6a infections while patients with liver disease could have acquired their infections earlier than volunteer blood donors and IDUs.
Among 393 patients with liver disease, a great diversity in HCV was detected, which reflects a constantly changing pattern of HCV genotypes in China over time.
我们最近在中国南方的志愿献血者和 IDU 中确定了 HCV 分离株，并揭示了基因型分布模式不仅在这两个研究队列之间不同，而且与我们在 2002 年采样的结果也不同。在肝病患者中，模式也可能发生了变化。
对 393 例肝病患者的 HCV E1 和 NS5B 序列进行了特征描述，并进行了系统进化分析。
鉴定出了 6 种 HCV 基因型、12 种亚型（1b：65.9%、6a：17.1%、2a：7.4%、3a：3.6%、3b：3.3%、6e：0.76%和 1a、1c、2b、2f、4d 和 5a 各 0.25%），并分类了两种新的 6 型 HCV 变体，这是迄今为止在中国发现的 HCV 最复杂的情况。尽管 1b 为主导，其次是 6a，与我们在 2002 年采样的情况基本一致，但首次报告了 1c、2f、4d、5a 和两种新的 HCV-6 变体的单个分离株。将 4d 从一名欧洲游客中排除后，所有其他患者均为中国患者。由于 6a 的比例（17.1%，67/393）低于我们最近在献血者（34.8%，82/236）和 IDU（51.5%，70/136）中检测到的比例，因此进行了进一步的统计分析。比较平均年龄表明，在 393 例患者中，感染 1b 的患者明显（6.7 岁）比感染 6a 的患者年龄大，而 393 例患者整体比我们最近报道的 236 名献血者（8.4 岁）和 136 名 IDU（12.6 岁）年龄大。解释是，年轻个体中 6a 感染的比例较高，而肝病患者的感染时间可能早于志愿献血者和 IDU。
在 393 例肝病患者中，检测到 HCV 的多样性很大，这反映了中国 HCV 基因型随时间不断变化的模式。
