Liu Q L, Williamson V M
Department of Nematology, University of California, Davis, CA 95616.
J Nematol. 2006 Mar;38(1):158-64.
Five isolates of M. hapla originating from the Netherlands and California were inbred by sequential transfer of single egg masses to produce six strains. Cytological examination showed that oocytes of these strains underwent meiosis and had n = 16 chromosomes. Strains were tested for ability to infect and to develop on several hosts by in vitro assays. The two strains from California infected tomato roots at a higher rate than those from the Netherlands, but no difference among strains was seen for ability to develop on tomato with or without the resistance gene Mi-1. All strains developed on the common bean cultivar Kentucky Wonder, but strains differed in ability to develop on the nematode-resistant cultivar NemaSnap. Strain-specific differences were also seen in ability to infect and to develop on Solanum bulbocastanum clone SB-22. Strain VW13, derived from nematodes treated with the mutagen EMS, was defective in ability to infect tomato and potato roots in vitro. Comparison of DNA using AFLP markers showed an average of 4% of the bands were polymorphic across the six strains, but no correlation was observed between the geographical origin or virulence and DNA polymorphism pattern.
从荷兰和加利福尼亚分离出的5株马铃薯金线虫通过连续转移单个卵块进行自交，产生了6个品系。细胞学检查表明，这些品系的卵母细胞进行减数分裂，有n = 16条染色体。通过体外试验检测了这些品系在几种寄主上的侵染和发育能力。来自加利福尼亚的两个品系侵染番茄根的速率高于来自荷兰的品系，但在有或没有抗性基因Mi-1的番茄上发育的能力在各品系间未观察到差异。所有品系都能在菜豆品种肯塔基奇观上发育，但在对线虫有抗性的品种NemaSnap上发育的能力各品系有所不同。在侵染和在马铃薯克隆SB-22上发育的能力方面也观察到品系特异性差异。源自用诱变剂EMS处理过的线虫的VW13品系在体外侵染番茄和马铃薯根的能力存在缺陷。使用AFLP标记进行DNA比较表明，六个品系中平均4%的条带具有多态性，但地理来源或毒力与DNA多态性模式之间未观察到相关性。
