Chen J, Moore W H, Yuen G Y, Kobayashi D, Caswell-Chen E P
Department of Nematology, University of California, Davis, CA 95616 Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583 Department of Plant Biology & Pathology, Rutgers, New Brunswick, NJ 08901-8520.
J Nematol. 2006 Jun;38(2):233-9.
Chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. Here, the influence of Lysobacter enzymogenes strain C3 on Caenorhabditis elegans, Heterodera schachtii, Meloidogyne javanica, Pratylenchus penetrans, and Aphelenchoides fragariae is described. Exposure of C. elegans to L. enzymogenes strain C3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched juveniles after 2 d. Hatch of H. schachtii eggs was about 50% on a lawn of L. enzymogenes strain C3 on agar as compared to 80% on a lawn of E. coli. Juveniles that hatched on a lawn of L. enzymogenes strain C3 on agar died due to disintegration of the cuticle and body contents. Meloidogyne javanica juveniles died after 4 d exposure to a 7-d-old chitin broth culture of L. enzymogenes strain C3. Immersion of A. fragariae, M. javanica, and P. penetrans juveniles and adults in a nutrient broth culture of L. enzymogenes strain C3 led to rapid death and disintegration of the nematodes. Upon exposure to L. enzymogenes strain C3 cultures in nutrient broth, H. schachtii juveniles were rapidly immobilized and then lysed after three days. The death and disintegration of the tested nematodes suggests that toxins and enzymes produced by this strain are active against a range of nematode species.
几丁质分解微生物群落可能通过降解卵壳导致卵活力下降，从而有助于对植物寄生线虫进行生物防治。在此，描述了产酶溶杆菌C3菌株对秀丽隐杆线虫、甜菜孢囊线虫、爪哇根结线虫、穿刺短体线虫和草莓滑刃线虫的影响。在琼脂上让秀丽隐杆线虫接触产酶溶杆菌C3菌株，2天后几乎完全抑制了产卵，且94%孵化出的幼虫死亡。与在大肠杆菌菌苔上相比，甜菜孢囊线虫卵在产酶溶杆菌C3菌株菌苔上的孵化率约为50%。在琼脂上产酶溶杆菌C3菌株菌苔上孵化出的幼虫因角质层和体内物质解体而死亡。爪哇根结线虫幼虫在接触产酶溶杆菌C3菌株7天的几丁质肉汤培养物4天后死亡。将草莓滑刃线虫、爪哇根结线虫和穿刺短体线虫的幼虫及成虫浸入产酶溶杆菌C3菌株的营养肉汤培养物中会导致线虫迅速死亡和解体。在营养肉汤中接触产酶溶杆菌C3菌株培养物后，甜菜孢囊线虫幼虫迅速被固定，3天后裂解。受试线虫的死亡和解体表明该菌株产生的毒素和酶对一系列线虫物种具有活性。
