Palomares Rius J E, Vovlas N, Troccoli A, Liébanas G, Landa B B, Castillo P
Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 4084, 14080 Córdoba, Spain Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche (C.N.R.), Sezione di Bari, Via G. Amendola 165/A, 70126 Bari, Italy Departmento de Biologia Animal, Biologia Vegetal y Ecología, Universidad de Jaén, Campus "Las Lagunillas" s/n, Edificio B3, 23071-Jaén, Spain.
J Nematol. 2007 Jun;39(2):190-202.
High infection rates of European sea rocket feeder roots by an unknown root-knot nematode were found in a coastal dune soil at Cullera (Valencia) in central eastern Spain. Morphometry, esterase and malate dehydrogenase electrophoretic phenotypes and phylogenetic trees demonstrated that this nematode species differs clearly from other previously described root-knot nematodes. Studies of host-parasite relationships showed a typical susceptible reaction in naturally infected European sea rocket plants and in artificially inoculated tomato (cv. Roma) and chickpea (cv. UC 27) plants. The species is herein described and illustrated and named as Meloidogyne dunensis n. sp. The new root-knot nematode can be distinguished from other Meloidogyne spp. by: (i) perineal pattern rounded-oval, formed of numerous fine dorsal and ventral cuticle striae and ridges, lateral fields clearly visible; (ii) female excretory pore at the level of stylet knobs, EP/ST ratio 1.6; (iii) second-stage juveniles with hemizonid located 1 to 2 annuli anteriorly to excretory pore and long, narrow, tapering tail; and (iv) males with lateral fields composed of four incisures anteriorly and posteriorly, while six distinct incisures are observed for large part at mid-body. Phylogenetic trees derived from distance and maximum parsimony analyses based on 18S, ITS1-5.8S-ITS2 and D2-D3 of 28S rDNA showed that M. dunensis n. sp. can be differentiated from all described root-knot nematode species, and it is clearly separated from other species with resemblance in morphology, such as M. duytsi, M. maritima, M. mayaguensis and M. minor.
在西班牙中东部瓦伦西亚库列拉的一处沿海沙丘土壤中，发现一种未知的根结线虫对欧洲海甘蓝的取食根具有很高的感染率。形态测量、酯酶和苹果酸脱氢酶电泳表型以及系统发育树表明，这种线虫物种与之前描述的其他根结线虫明显不同。宿主 - 寄生虫关系研究表明，在自然感染的欧洲海甘蓝植株以及人工接种的番茄（品种为罗马）和鹰嘴豆（品种为UC 27）植株中出现了典型的感病反应。在此对该物种进行了描述、图示并命名为海滨根结线虫新种（Meloidogyne dunensis n. sp.）。这种新的根结线虫可通过以下特征与其他根结线虫属物种区分开来：（i）会阴花纹呈圆形 - 椭圆形，由许多细小的背侧和腹侧角质层条纹及脊形成，侧区清晰可见；（ii）雌虫排泄孔位于口针基部水平，排泄孔与口针长度比为1.6；（iii）二龄幼虫的半侧线位于排泄孔前方1至2个环纹处，尾部细长且逐渐变细；（iv）雄虫的侧区在前后部由四条刻线组成，而在虫体中部大部分区域观察到六条明显的刻线。基于28S rDNA的18S、ITS1 - 5.8S - ITS2和D2 - D3进行距离分析和最大简约分析得出的系统发育树表明，海滨根结线虫新种可与所有已描述的根结线虫物种区分开来，并且与形态相似的其他物种如杜氏根结线虫（M. duytsi）、滨海根结线虫（M. maritima）、马亚圭根结线虫（M. mayaguensis）和微小根结线虫（M. minor）明显分开。
